Protein-carbohydrate complex reveals circulating metastatic cells in a microfluidic assay
Malara, Natalia Maria
Di Fabrizio, Enzo M.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Material Science and Engineering Program
Physical Science and Engineering (PSE) Division
Online Publication Date2013-02-11
Print Publication Date2013-06-24
Permanent link to this recordhttp://hdl.handle.net/10754/562651
MetadataShow full item record
AbstractAdvances in carbohydrate sequencing technologies reveal the tremendous complexity of the glycome and the role that glycomics might have to bring insight into the biological functions. Carbohydrate-protein interactions, in particular, are known to be crucial to most mammalian physiological processes as mediators of cell adhesion and metastasis, signal transducers, and organizers of protein interactions. An assay is developed here to mimic the multivalency of biological complexes that selectively and sensitively detect carbohydrate-protein interactions. The binding of β-galactosides and galectin-3 - a protein that is correlated to the progress of tumor and metastasis - is examined. The efficiency of the assay is related to the expression of the receptor while anchoring to the interaction's strength. Comparative binding experiments reveal molecular binding preferences. This study establishes that the assay is robust to isolate metastatic cells from colon affected patients and paves the way to personalized medicine. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
CitationSimone, G., Malara, N., Trunzo, V., Perozziello, G., Neuzil, P., Francardi, M., … Di Fabrizio, E. (2013). Protein-Carbohydrate Complex Reveals Circulating Metastatic Cells in a Microfluidic Assay. Small, 9(12), 2152–2161. doi:10.1002/smll.201202867
SponsorsThis work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti." (cod. PON01_02782, CUP B71H11000870005), and the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro, the foundation "T. Campanella" and RSA (ANASTE Calabria).
- Cantilever array sensors detect specific carbohydrate-protein interactions with picomolar sensitivity.
- Authors: Gruber K, Horlacher T, Castelli R, Mader A, Seeberger PH, Hermann BA
- Issue date: 2011 May 24
- Are matrix-immobilized neoglycoproteins, plant and human lectins and carbohydrate--binding antibodies from human serum mediators of adhesion in vitro for carcinoma and lymphosarcoma cells?
- Authors: Wawotzny R, André S, Dong X, Joshi SS, Gabius HJ
- Issue date: 1995 Jan-Feb
- Colon cancer mucin: a new ligand for the beta-galactoside-binding protein galectin-3.
- Authors: Bresalier RS, Byrd JC, Wang L, Raz A
- Issue date: 1996 Oct 1
- Peptides specific to the galectin-3 carbohydrate recognition domain inhibit metastasis-associated cancer cell adhesion.
- Authors: Zou J, Glinsky VV, Landon LA, Matthews L, Deutscher SL
- Issue date: 2005 Feb
- [Cancer-associated carbohydrate-binding protein galectin-3 and its therapeutic ligands].
- Authors: Bai H, Liu L, Li YH, You Y, Mei QB
- Issue date: 2008 Apr