Reactor performance in terms of COD and nitrogen removal and bacterial community structure of a three-stage rotating bioelectrochemical contactor
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Biotechnology Research Group
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
Permanent link to this recordhttp://hdl.handle.net/10754/562624
MetadataShow full item record
AbstractIntegrating microbial fuel cell (MFC) into rotating biological contactor (RBC) creates an opportunity for enhanced removal of COD and nitrogen coupled with energy generation from wastewater. In this study, a three-stage rotating bioelectrochemical contactor (referred to as RBC-MFC unit) integrating MFC with RBC technology was constructed for simultaneous removal of carbonaceous and nitrogenous compounds and electricity generation from a synthetic medium containing acetate and ammonium. The performance of the RBC-MFC unit was compared to a control reactor (referred to as RBC unit) that was operated under the same conditions but without current generation (i.e. open-circuit mode). The effect of hydraulic loading rate (HLR) and COD/N ratio on the performance of the two units was investigated. At low (3.05 gCOD g-1N) and high COD/N ratio (6.64 gCOD g-1N), both units achieved almost similar COD and ammonia-nitrogen removal. However, the RBC-MFC unit achieved significantly higher denitrification and nitrogen removal compared to the RBC unit indicating improved denitrification at the cathode due to current flow. The average voltage under 1000 Ω external resistance ranged between 0.03 and 0.30 V and between 0.02 and 0.21 V for stages 1 and 2 of the RBC-MFC unit. Pyrosequencing analysis of bacterial 16S rRNA gene revealed high bacterial diversity at the anode and cathode of both units. Genera that play a role in nitrification (Nitrospira; Nitrosomonas), denitrification (Comamonas; Thauera) and electricity generation (Geobacter) were identified at the electrodes. Geobacter was only detected on the anode of the RBC-MFC unit. Nitrifiers and denitrifiers were more abundant in the RBC-MFC unit compared to the RBC unit and were largely present on the cathode of both units suggesting that most of the nitrogen removal occurred at the cathode. © 2012 Elsevier Ltd.
CitationSayess, R. R., Saikaly, P. E., El-Fadel, M., Li, D., & Semerjian, L. (2013). Reactor performance in terms of COD and nitrogen removal and bacterial community structure of a three-stage rotating bioelectrochemical contactor. Water Research, 47(2), 881–894. doi:10.1016/j.watres.2012.11.023
SponsorsThis research was supported by discretionary investigator funds (P.S.) at King Abdullah University of Science and Technology (KAUST). The material presented is also based in part upon work supported by the University Research Board at the American University of Beirut. Special thanks are extended to Mohammed Alarawi and Shahjahan Ali at the Bioscience Core Laboratory at KAUST.
- Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454 high-throughput pyrosequencing.
- Authors: Ma J, Wang Z, Yang Y, Mei X, Wu Z
- Issue date: 2013 Feb 1
- Oxygen-limited nitrification and denitrification in a lab-scale rotating biological contactor.
- Authors: Pynaert K, Sprengers R, Laenen J, Verstraete W
- Issue date: 2002 Mar
- Simultaneous phenol removal, nitrification and denitrification using microbial fuel cell technology.
- Authors: Feng C, Huang L, Yu H, Yi X, Wei C
- Issue date: 2015 Jun 1
- Effect of mean cell residence time on the performance and microbial diversity of pre-denitrification submerged membrane bioreactors.
- Authors: Tan TW, Ng HY, Ong SL
- Issue date: 2008 Jan
- Organic and nitrogen removal in a two-stage rotating biological contactor treating municipal wastewater.
- Authors: Hiras DN, Manariotis ID, Grigoropoulos SG
- Issue date: 2004 May