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dc.contributor.authorMoon, Robert W.
dc.contributor.authorHall, Joanna M.
dc.contributor.authorRangkuti, Farania
dc.contributor.authorHo, YungShwen
dc.contributor.authorAlmond, Neil M.
dc.contributor.authorMitchell, Graham Howard
dc.contributor.authorPain, Arnab
dc.contributor.authorHolder, Anthony A.
dc.contributor.authorBlackman, Michael J.
dc.date.accessioned2015-08-03T10:39:14Z
dc.date.available2015-08-03T10:39:14Z
dc.date.issued2012-12-24
dc.identifier.issn00278424
dc.identifier.pmid23267069
dc.identifier.doi10.1073/pnas.1216457110
dc.identifier.urihttp://hdl.handle.net/10754/562466
dc.description.abstractResearch into the aetiological agent of the most widespread form of severe malaria, Plasmodium falciparum, has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct Plasmodium species, Plasmodium vivax, and it has become increasingly apparent that zoonotic infection by the closely related simian parasite Plasmodium knowlesi is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of P. knowlesi to continuous culture in human erythrocytes. Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.
dc.description.sponsorshipThis work was supported by the United Kingdom Medical Research Council (U117532063 and U117532067), the European Community's FP7 Programme under Grant Agreement 242095 (EviMalar), and a Medical Research Council Career Development fellowship (to R. W. M.). A. P. was funded by his faculty baseline support and Office for Competitive Research Funds from the King Abdullah University of Science and Technology.
dc.publisherProceedings of the National Academy of Sciences
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545754
dc.subjectInvasion
dc.subjectTransfection
dc.titleAdaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes
dc.typeArticle
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputer Science Program
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalProceedings of the National Academy of Sciences
dc.identifier.pmcidPMC3545754
dc.contributor.institutionNatl Inst Med Res, MRC, Div Parasitol, London NW7 1AA, England
dc.contributor.institutionHlth Protect Agcy, Natl Inst Biol Stand & Controls, Div Retrovirol, Potters Bar EN6 3QG, Herts, England
dc.contributor.institutionGuys Hosp, Kings Hosp, Sch Med Guys, Peter Gorer Dept Immunobiol,Kings Coll London, London SE1 9RT, England
dc.contributor.institutionGuys Hosp, St Thomas Hosp, London SE1 9RT, England
kaust.personRangkuti, Farania
kaust.personPain, Arnab
kaust.personHo, YungShwen
dc.date.published-online2012-12-24
dc.date.published-print2013-01-08


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