Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes
AuthorsMoon, Robert W.
Hall, Joanna M.
Almond, Neil M.
Mitchell, Graham Howard
Holder, Anthony A.
Blackman, Michael J.
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Computer Science Program
Pathogen Genomics Laboratory
Online Publication Date2012-12-24
Print Publication Date2013-01-08
Permanent link to this recordhttp://hdl.handle.net/10754/562466
MetadataShow full item record
AbstractResearch into the aetiological agent of the most widespread form of severe malaria, Plasmodium falciparum, has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct Plasmodium species, Plasmodium vivax, and it has become increasingly apparent that zoonotic infection by the closely related simian parasite Plasmodium knowlesi is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of P. knowlesi to continuous culture in human erythrocytes. Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.
CitationMoon, R. W., Hall, J., Rangkuti, F., Ho, Y. S., Almond, N., Mitchell, G. H., … Blackman, M. J. (2012). Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes. Proceedings of the National Academy of Sciences, 110(2), 531–536. doi:10.1073/pnas.1216457110
SponsorsThis work was supported by the United Kingdom Medical Research Council (U117532063 and U117532067), the European Community's FP7 Programme under Grant Agreement 242095 (EviMalar), and a Medical Research Council Career Development fellowship (to R. W. M.). A. P. was funded by his faculty baseline support and Office for Competitive Research Funds from the King Abdullah University of Science and Technology.
PubMed Central IDPMC3545754
- Human red blood cell-adapted Plasmodium knowlesi parasites: a new model system for malaria research.
- Authors: Grüring C, Moon RW, Lim C, Holder AA, Blackman MJ, Duraisingh MT
- Issue date: 2014 May
- A new single-step PCR assay for the detection of the zoonotic malaria parasite Plasmodium knowlesi.
- Authors: Lucchi NW, Poorak M, Oberstaller J, DeBarry J, Srinivasamoorthy G, Goldman I, Xayavong M, da Silva AJ, Peterson DS, Barnwell JW, Kissinger J, Udhayakumar V
- Issue date: 2012
- Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections.
- Authors: Lee KS, Cox-Singh J, Singh B
- Issue date: 2009 Apr 21
- Comparison of the susceptibility of Plasmodium knowlesi and Plasmodium falciparum to antimalarial agents.
- Authors: van Schalkwyk DA, Moon RW, Blasco B, Sutherland CJ
- Issue date: 2017 Nov 1
- Cellular dissection of malaria parasite invasion of human erythrocytes using viable Plasmodium knowlesi merozoites.
- Authors: Lyth O, Vizcay-Barrena G, Wright KE, Haase S, Mohring F, Najer A, Henshall IG, Ashdown GW, Bannister LH, Drew DR, Beeson JG, Fleck RA, Moon RW, Wilson DW, Baum J
- Issue date: 2018 Jul 5