Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionCoastal and Marine Resources Core Lab
Marine Science Program
Red Sea Research Center (RSRC)
Reef Ecology Lab
Date
2012-12-18Online Publication Date
2012-12-18Print Publication Date
2013-06Permanent link to this record
http://hdl.handle.net/10754/562460
Metadata
Show full item recordAbstract
To investigate population genetic patterns of the anemonefish Amphiprion bicinctus in the Red Sea, we isolated and characterized 35 microsatellite loci using 454-sequencing. Microsatellite sequences were identified using the Tandem Repeats Finder program. The 35 loci were tested on 80 individuals sampled from two spatially separated populations along the Saudi Arabian Red Sea coast. We found a mean of 10. 9 alleles per locus and observed levels of heterozygosity ranged from 0. 4 to 0. 98. All loci were polymorphic, none deviated significantly from Hardy-Weinberg equilibrium, no linkage disequilibrium was observed and there was no evidence for -alleles in both populations. The markers reported here constitute the first specific set for this species, and they are expected to contribute to future studies of connectivity in the Red Sea region. © 2012 Springer Science+Business Media Dordrecht.Citation
Nanninga, G., Mughal, M., Saenz-Agudelo, P., Bayer, T., & Berumen, M. (2012). Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus. Conservation Genetics Resources, 5(2), 515–518. doi:10.1007/s12686-012-9841-0Publisher
Springer NatureJournal
Conservation Genetics Resourcesae974a485f413a2113503eed53cd6c53
10.1007/s12686-012-9841-0