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    Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus

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    Type
    Article
    Authors
    Nanninga, Gerrit B. cc
    Mughal, Mehreen
    Saenz Agudelo, Pablo
    Bayer, Till
    Berumen, Michael L. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Coastal and Marine Resources Core Lab
    Marine Science Program
    Red Sea Research Center (RSRC)
    Reef Ecology Lab
    Date
    2012-12-18
    Online Publication Date
    2012-12-18
    Print Publication Date
    2013-06
    Permanent link to this record
    http://hdl.handle.net/10754/562460
    
    Metadata
    Show full item record
    Abstract
    To investigate population genetic patterns of the anemonefish Amphiprion bicinctus in the Red Sea, we isolated and characterized 35 microsatellite loci using 454-sequencing. Microsatellite sequences were identified using the Tandem Repeats Finder program. The 35 loci were tested on 80 individuals sampled from two spatially separated populations along the Saudi Arabian Red Sea coast. We found a mean of 10. 9 alleles per locus and observed levels of heterozygosity ranged from 0. 4 to 0. 98. All loci were polymorphic, none deviated significantly from Hardy-Weinberg equilibrium, no linkage disequilibrium was observed and there was no evidence for -alleles in both populations. The markers reported here constitute the first specific set for this species, and they are expected to contribute to future studies of connectivity in the Red Sea region. © 2012 Springer Science+Business Media Dordrecht.
    Citation
    Nanninga, G., Mughal, M., Saenz-Agudelo, P., Bayer, T., & Berumen, M. (2012). Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus. Conservation Genetics Resources, 5(2), 515–518. doi:10.1007/s12686-012-9841-0
    Publisher
    Springer Nature
    Journal
    Conservation Genetics Resources
    DOI
    10.1007/s12686-012-9841-0
    ae974a485f413a2113503eed53cd6c53
    10.1007/s12686-012-9841-0
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Red Sea Research Center (RSRC); Marine Science Program; Coastal and Marine Resources Core Lab

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