Spatiotemporal visualization of subcellular dynamics of carbon nanotubes
Type
ArticleAuthors
Serag, Maged F.
Braeckmans, Kevin
Habuchi, Satoshi

Kaji, Noritada
Bianco, Alberto
Baba, Yoshinobu
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Single-Molecule Spectroscopy and Microscopy Research Group
Date
2012-11-28Online Publication Date
2012-11-28Print Publication Date
2012-12-12Permanent link to this record
http://hdl.handle.net/10754/562456
Metadata
Show full item recordAbstract
To date, there is no consensus on the relationship between the physicochemical characteristics of carbon nanotubes (CNTs) and their biological behavior; however, there is growing evidence that the versatile characteristics make their biological fate largely unpredictable and remain an issue of limited knowledge. Here we introduce an experimental methodology for tracking and visualization of postuptake behavior and the intracellular fate of CNTs based on the spatial distribution of diffusion values throughout the plant cell. By using raster scan image correlation spectroscopy (RICS), we were able to generate highly quantitative spatial maps of CNTs diffusion in different cell compartments. The spatial map of diffusion values revealed that the uptake of CNTs is associated with important subcellular events such as carrier-mediated vacuolar transport and autophagy. These results show that RICS is a useful methodology to elucidate the intracellular behavior mechanisms of carbon nanotubes and potentially other fluorescently labeled nanoparticles, which is of relevance for the important issues related to the environmental impact and health hazards. © 2012 American Chemical Society.Citation
Serag, M. F., Braeckmans, K., Habuchi, S., Kaji, N., Bianco, A., & Baba, Y. (2012). Spatiotemporal Visualization of Subcellular Dynamics of Carbon Nanotubes. Nano Letters, 12(12), 6145–6151. doi:10.1021/nl3029625Sponsors
This work was partly supported by Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. This research was partly supported by the Industrial Technology Research Grant Program from the New Energy and Industrial Technology Development Organization (NEDO) of Japan and the Japan Society for the Promotion of Science (JSPS) through its "Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)". M.F.S. wishes to extend a sincere gratitude to Prof. Jason R. Dwyer at University of Rhode Island (USA) for his valuable contributions during writing the manuscript. A.B. wishes to thank CNRS and JSPS for the invited fellowship in the framework of the Invitation Fellowship Program for Research in Japan (ID No. S-12072). The authors wish to thank Prof. Marcel Ameloot and Dr. Ben De Clercq at Hasselt University (Belgium) and Dr. Frederic Gros at University of Strasbourg (France) for helpful discussions and comments. The fluorescence data were analyzed using the Globals software package developed at the Laboratory for Fluorescence Dynamics at the University of Illinois at Urbana-Champaign. The experimental work and data analysis were done in the Department of Applied Chemistry, Nagoya University, Japan.Publisher
American Chemical Society (ACS)Journal
Nano LettersPubMed ID
23170917ae974a485f413a2113503eed53cd6c53
10.1021/nl3029625
Scopus Count
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