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dc.contributor.authorFurumaki, Shu
dc.contributor.authorYabiku, Yu
dc.contributor.authorHabuchi, Satoshi
dc.contributor.authorTsukatani, Yusuke
dc.contributor.authorBryant, Donald A.
dc.contributor.authorVácha, Martin
dc.date.accessioned2015-08-03T10:38:46Z
dc.date.available2015-08-03T10:38:46Z
dc.date.issued2012-11-20
dc.identifier.citationFurumaki, S., Yabiku, Y., Habuchi, S., Tsukatani, Y., Bryant, D. A., & Vacha, M. (2012). Circular Dichroism Measured on Single Chlorosomal Light-Harvesting Complexes of Green Photosynthetic Bacteria. The Journal of Physical Chemistry Letters, 3(23), 3545–3549. doi:10.1021/jz301671p
dc.identifier.issn19487185
dc.identifier.pmid26290985
dc.identifier.doi10.1021/jz301671p
dc.identifier.urihttp://hdl.handle.net/10754/562454
dc.description.abstractWe report results on circular dichroism (CD) measured on single immobilized chlorosomes of a triple mutant of green sulfur bacterium Chlorobaculum tepidum. The CD signal is measured by monitoring chlorosomal bacteriochlorphyll c fluorescence excited by alternate left and right circularly polarized laser light with a fixed wavelength of 733 nm. The excitation wavelength is close to a maximum of the negative CD signal of a bulk solution of the same chlorosomes. The average CD dissymmetry parameter obtained from an ensemble of individual chlorosomes was gs = -0.025, with an intrinsic standard deviation (due to variations between individual chlorosomes) of 0.006. The dissymmetry value is about 2.5 times larger than that obtained at the same wavelength in the bulk solution. The difference can be satisfactorily explained by taking into account the orientation factor in the single-chlorosome experiments. The observed distribution of the dissymmetry parameter reflects the well-ordered nature of the mutant chlorosomes. © 2012 American Chemical Society.
dc.description.sponsorshipThe work was supported by a Grant-in-Aid for Scientific Research No. 23651107 of the Japan Society for the Promotion of Science (M.V.).
dc.publisherAmerican Chemical Society (ACS)
dc.titleCircular dichroism measured on single chlorosomal light-harvesting complexes of green photosynthetic bacteria
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentSingle-Molecule Spectroscopy and Microscopy Research Group
dc.identifier.journalThe Journal of Physical Chemistry Letters
dc.contributor.institutionDepartment of Organic and Polymeric Materials, Tokyo Institute of Technology, Ookayama 2-12-1-S8, Meguro-ku, Tokyo 152-8552, Japan
dc.contributor.institutionDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, United States
dc.contributor.institutionDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, MN 59717, United States
dc.contributor.institutionGraduate School of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan
dc.contributor.institutionPRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan
kaust.personHabuchi, Satoshi
dc.date.published-online2012-11-20
dc.date.published-print2012-12-06


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