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    Arabidopsis plastid AMOS1/EGY1 integrates abscisic acid signaling to regulate global gene expression response to ammonium stress

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    Type
    Article
    Authors
    Li, Baohai
    Li, Qing
    Xiong, Liming cc
    Kronzucker, Herbert J.
    Krämer, Ute
    Shi, Weiming
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Desert Agriculture Initiative
    Bioscience Program
    Plant Stress Genomics Research Lab
    Date
    2012-10-12
    Online Publication Date
    2012-10-12
    Print Publication Date
    2012-12-01
    Permanent link to this record
    http://hdl.handle.net/10754/562364
    
    Metadata
    Show full item record
    Abstract
    Ammonium (NH4 +) is a ubiquitous intermediate of nitrogen metabolism but is notorious for its toxic effects on most organisms. Extensive studies of the underlying mechanisms of NH4 + toxicity have been reported in plants, but it is poorly understood how plants acclimate to high levels of NH4 +. Here, we identified an Arabidopsis (Arabidopsis thaliana) mutant, ammonium overly sensitive1 (amos1), that displays severe chlorosis under NH4 + stress. Map-based cloning shows amos1 to carry a mutation in EGY1 (for ethylene-dependent, gravitropism-deficient, and yellow-green-like protein1), which encodes a plastid metalloprotease. Transcriptomic analysis reveals that among the genes activated in response to NH4 +, 90% are regulated dependent on AMOS1/ EGY1. Furthermore, 63% of AMOS1/EGY1-dependent NH4 +-activated genes contain an ACGTG motif in their promoter region, a core motif of abscisic acid (ABA)-responsive elements. Consistent with this, our physiological, pharmacological, transcriptomic, and genetic data show that ABA signaling is a critical, but not the sole, downstream component of the AMOS1/EGY1-dependent pathway that regulates the expression of NH4 +-responsive genes and maintains chloroplast functionality under NH4 + stress. Importantly, abi4 mutants defective in ABA-dependent and retrograde signaling, but not ABA-deficient mutants, mimic leaf NH4 + hypersensitivity of amos1. In summary, our findings suggest that an NH4 +-responsive plastid retrograde pathway, which depends on AMOS1/EGY1 function and integrates with ABA signaling, is required for the regulation of expression of the presence of high NH4 + levels. © 2012 American Society of Plant Biologists. All Rights Reserved.
    Sponsors
    This work was supported by the National Science Foundation of China (grant nos. 31200189 and 30771285), the Chinese Academy Sciences Innovation Program (grant no. ISSASIP1103), the National Sciences and Engineering Research Council of Canada (grant no. 217277-2009), and the National Nature Science Foundation (grant nos. 91017013 and 31070327).
    Publisher
    American Society of Plant Biologists (ASPB)
    Journal
    PLANT PHYSIOLOGY
    DOI
    10.1104/pp.112.206508
    PubMed ID
    23064408
    PubMed Central ID
    PMC3510130
    Additional Links
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510130
    ae974a485f413a2113503eed53cd6c53
    10.1104/pp.112.206508
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Desert Agriculture Initiative

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