Deep Sequencing of Myxilla (Ectyomyxilla) methanophila, an Epibiotic Sponge on Cold-Seep Tubeworms, Reveals Methylotrophic, Thiotrophic, and Putative Hydrocarbon-Degrading Microbial Associations
AuthorsArellano, Shawn M.
Lafi, Feras Fawzi
KAUST DepartmentCenter for Desert Agriculture
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division
KAUST Global Collaborative Research Program
Online Publication Date2012-10-11
Print Publication Date2013-02
Permanent link to this recordhttp://hdl.handle.net/10754/562363
MetadataShow full item record
AbstractThe encrusting sponge Myxilla (Ectyomyxilla) methanophila (Poecilosclerida: Myxillidae) is an epibiont on vestimentiferan tubeworms at hydrocarbon seeps on the upper Louisiana slope of the Gulf of Mexico. It has long been suggested that this sponge harbors methylotrophic bacteria due to its low δ13C value and high methanol dehydrogenase activity, yet the full community of microbial associations in M. methanophila remained uncharacterized. In this study, we sequenced 16S rRNA genes representing the microbial community in M. methanophila collected from two hydrocarbon-seep sites (GC234 and Bush Hill) using both Sanger sequencing and next-generation 454 pyrosequencing technologies. Additionally, we compared the microbial community in M. methanophila to that of the biofilm collected from the associated tubeworm. Our results revealed that the microbial diversity in the sponges from both sites was low but the community structure was largely similar, showing a high proportion of methylotrophic bacteria of the genus Methylohalomonas and polycyclic aromatic hydrocarbon (PAH)-degrading bacteria of the genera Cycloclasticus and Neptunomonas. Furthermore, the sponge microbial clone library revealed the dominance of thioautotrophic gammaproteobacterial symbionts in M. methanophila. In contrast, the biofilm communities on the tubeworms were more diverse and dominated by the chemoorganotrophic Moritella at GC234 and methylotrophic Methylomonas and Methylohalomonas at Bush Hill. Overall, our study provides evidence to support previous suggestion that M. methanophila harbors methylotrophic symbionts and also reveals the association of PAH-degrading and thioautotrophic microbes in the sponge. © 2012 Springer Science+Business Media New York.
CitationArellano, S. M., Lee, O. O., Lafi, F. F., Yang, J., Wang, Y., Young, C. M., & Qian, P.-Y. (2012). Deep Sequencing of Myxilla (Ectyomyxilla) methanophila, an Epibiotic Sponge on Cold-Seep Tubeworms, Reveals Methylotrophic, Thiotrophic, and Putative Hydrocarbon-Degrading Microbial Associations. Microbial Ecology, 65(2), 450–461. doi:10.1007/s00248-012-0130-y
SponsorsWe gratefully acknowledge Mr. Rick Webb, at the Centre for Microscopy and Microanalysis (University of Queensland), who performed the TEM. We also thank Dr. Salim Bougouffa (Hong Kong University of Science and Technology) for the useful advice in bioinformatics and data analysis. Ship time and submersible dives were funded by grant number OCE-0527139 from the National Science Foundation to CMY. This research was supported by the National Basic Research Program of China (973 Program, No. 2012CB417304) and an award (SA-C0040/UK-C0016) made by the King Abdullah University of Science and Technology to PYQ. SMA was supported by a Woods Hole Oceanographic Institution postdoctoral scholarship during manuscript preparation.
- Association of thioautotrophic bacteria with deep-sea sponges.
- Authors: Nishijima M, Lindsay DJ, Hata J, Nakamura A, Kasai H, Ise Y, Fisher CR, Fujiwara Y, Kawato M, Maruyama T
- Issue date: 2010 Jun
- Bacterial symbionts of Bathymodiolus mussels and Escarpia tubeworms from Chapopote, an asphalt seep in the Southern Gulf of Mexico.
- Authors: Raggi L, Schubotz F, Hinrichs KU, Dubilier N, Petersen JM
- Issue date: 2013 Jul
- Pyrosequencing reveals diverse and distinct sponge-specific microbial communities in sponges from a single geographical location in Irish waters.
- Authors: Jackson SA, Kennedy J, Morrissey JP, O'Gara F, Dobson AD
- Issue date: 2012 Jul
- Phylogenetic diversity, host-specificity and community profiling of sponge-associated bacteria in the northern Gulf of Mexico.
- Authors: Erwin PM, Olson JB, Thacker RW
- Issue date: 2011
- Enrichment versus biofilm culture: a functional and phylogenetic comparison of polycyclic aromatic hydrocarbon-degrading microbial communities.
- Authors: Stach JE, Burns RG
- Issue date: 2002 Mar