Show simple item record

dc.contributor.authorIdris, Ali
dc.contributor.authorAbdullah, N.
dc.contributor.authorBrown, Judith K.
dc.date.accessioned2015-08-03T10:01:44Z
dc.date.available2015-08-03T10:01:44Z
dc.date.issued2012-10
dc.identifier.citationIdris, A. M., Abdullah, N. M., & Brown, J. K. (2012). Leaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellites. Virus Research, 169(1), 296–300. doi:10.1016/j.virusres.2012.07.014
dc.identifier.issn01681702
dc.identifier.pmid22841489
dc.identifier.doi10.1016/j.virusres.2012.07.014
dc.identifier.urihttp://hdl.handle.net/10754/562344
dc.description.abstractThe complete genome of 2780 bases was amplified using rolling circle amplification, and cloned, and sequenced for two distinct strains of the monopartite begomovirus Tomato leaf curl Sudan virus (ToLCSDV). The two strains shared 86-91% identity with the previously described ToLCSDV from the Nile Basin, and 90-91% identity with one another. One strain was cloned from symptomatic tomato plants from Tihamah (ToLCSDV-YE[YE:Tih:05]) while the other was cloned from symptomatic tobacco plants collected from Wadi Hadramaut (ToLCSDV-YE[YE:Had:89]). A distinct full-length betasatellite molecule (1352 bases) was cloned from the respective field-infected tomato and tobacco plants. Agro-inoculation of tomato and Nicotiana benthamiana plants with cloned partial tandem repeats of ToLCSDV-YE[YE:Tih11:05]) and the associated betasatellite, Tomato leaf curl Yemen betasatellite (ToLCYEB-[Tih:tom:137:05]), resulted in the reproduction of leaf curl disease symptoms in test plants like those observed in the field-infected plants. The betasatellite contributed to symptom severity in N. benthamiana test plants when it was co-inoculated with ToLCSDV-YE, compared to the milder symptoms that were observed in tobacco plants infected with the helper virus alone. © 2012 Elsevier B.V.
dc.description.sponsorshipThis research was supported in part by a SABIC Fellowship to the first author, and a Fulbright scholarship to the second author.
dc.publisherElsevier BV
dc.subjectGeminiviridae
dc.subjectKoch's postulates
dc.subjectTobacco
dc.subjectTomato virus
dc.titleLeaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellites
dc.typeArticle
dc.contributor.departmentCenter for Desert Agriculture
dc.identifier.journalVirus Research
dc.contributor.institutionUniv Arizona, Sch Plant Sci, Tucson, AZ 85721 USA
dc.contributor.institutionSanaa Univ, Dept Plant Protect, Sanaa, Yemen
kaust.personIdris, Ali


This item appears in the following Collection(s)

Show simple item record