Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds
AuthorsGerhardt, Lutz Christian
Widdows, Kate L.
Erol, Melek M.
Roqan, Iman S.
Ansari, Tahera I.
Boccaccini, Aldo R.
KAUST DepartmentMaterial Science and Engineering Program
Physical Science and Engineering (PSE) Division
Semiconductor and Material Spectroscopy (SMS) Laboratory
Online Publication Date2012-09-11
Print Publication Date2013-03
Permanent link to this recordhttp://hdl.handle.net/10754/562325
MetadataShow full item record
AbstractIn this study, the in vivo recellularization and neovascularization of nanosized bioactive glass (n-BG)-coated decellu-larized trabecular bone scaffolds were studied in a rat model and quantified using stereological analyses. Based on the highest amount of vascular endothelial growth factor (VEGF) secreted by human fibroblasts grown on n-BG coatings (0-1.245 mg/cm 2), decellularized trabecular bone samples (porosity: 43-81%) were coated with n-BG particles. Grown on n-BG particles at a coating density of 0.263 mg/cm2, human fibroblasts produced 4.3 times more VEGF than on uncoated controls. After 8 weeks of implantation in Sprague-Dawley rats, both uncoated and n-BG-coated samples were well infiltrated with newly formed tissue (47-48%) and blood vessels (3-4%). No significant differences were found in cellularization and vascularization between uncoated bone scaffolds and n-BG-coated scaffolds. This finding indicates that the decellularized bone itself may exhibit growth-promoting properties induced by the highly interconnected pore microarchitecture and/or proteins left behind on decellularized scaffolds. Even if we did not find proangiogenic effects in n-BG-coated bone scaffolds, a bioactive coating is considered to be beneficial to impart osteoinductive and osteoconductive properties to decellularized bone. n-BG-coated bone grafts have thus high clinical potential for the regeneration of complex tissue defects given their ability for recellularization and neovascularization. © 2012 Wiley Periodicals, Inc.
CitationGerhardt, L.-C., Widdows, K. L., Erol, M. M., Nandakumar, A., Roqan, I. S., Ansari, T., & Boccaccini, A. R. (2012). Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds. Journal of Biomedical Materials Research Part A, 101A(3), 827–841. doi:10.1002/jbm.a.34373
SponsorsContract grant sponsors: Partial funding by King Abdullah University of Science and Technology (KAUST; 2009-2010)
- The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds.
- Authors: Gerhardt LC, Widdows KL, Erol MM, Burch CW, Sanz-Herrera JA, Ochoa I, Stämpfli R, Roqan IS, Gabe S, Ansari T, Boccaccini AR
- Issue date: 2011 Jun
- Effect of bioactive borate glass microstructure on bone regeneration, angiogenesis, and hydroxyapatite conversion in a rat calvarial defect model.
- Authors: Bi L, Rahaman MN, Day DE, Brown Z, Samujh C, Liu X, Mohammadkhah A, Dusevich V, Eick JD, Bonewald LF
- Issue date: 2013 Aug
- Preparation and in vitro characterization of electrospun PVA scaffolds coated with bioactive glass for bone regeneration.
- Authors: Gao C, Gao Q, Li Y, Rahaman MN, Teramoto A, Abe K
- Issue date: 2012 May
- New approach to bone tissue engineering: simultaneous application of hydroxyapatite and bioactive glass coated on a poly(L-lactic acid) scaffold.
- Authors: Dinarvand P, Seyedjafari E, Shafiee A, Jandaghi AB, Doostmohammadi A, Fathi MH, Farhadian S, Soleimani M
- Issue date: 2011 Nov
- Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration.
- Authors: Leach JK, Kaigler D, Wang Z, Krebsbach PH, Mooney DJ
- Issue date: 2006 Jun