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    IAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress

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    Type
    Article
    Authors
    Kinoshita, Natsuko
    Wang, Huan
    Kasahara, Hiroyuki
    Liu, Jun
    MacPherson, Cameron R.
    Machida, Yasunori
    Kamiya, Yuji
    Hannah, Matthew A.
    Chuaa, Nam Hai
    KAUST Department
    Computational Bioscience Research Center (CBRC)
    Bioscience Program
    Date
    2012-09-07
    Online Publication Date
    2012-09-07
    Print Publication Date
    2012-09-01
    Permanent link to this record
    http://hdl.handle.net/10754/562315
    
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    Abstract
    The functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles of stress-responsive microRNAs and their targets are not as well understood. Using small RNA deep sequencing and ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) as a new target of miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site and under high osmotic stress miR167a levels decreased, whereas IAR3 mRNA levels increased. IAR3 hydrolyzes an inactive form of auxin (indole-3-acetic acid [IAA]-alanine) and releases bioactive auxin (IAA), a central phytohormone for root development. In contrast with the wild type, iar3 mutants accumulated reduced IAA levels and did not display high osmotic stress-induced root architecture changes. Transgenic plants expressing a cleavage-resistant form of IAR3 mRNA accumulated high levels of IAR3 mRNAs and showed increased lateral root development compared with transgenic plants expressing wild-type IAR3. Expression of an inducible noncoding RNA to sequester miR167a by target mimicry led to an increase in IAR3 mRNA levels, further confirming the inverse relationship between the two partners. Sequence comparison revealed the miR167 target site on IAR3 mRNA is conserved in evolutionarily distant plant species. Finally, we showed that IAR3 is required for drought tolerance. © 2012 American Society of Plant Biologists. All rights reserved.
    Citation
    Kinoshita, N., Wang, H., Kasahara, H., Liu, J., MacPherson, C., Machida, Y., … Chua, N.-H. (2012). IAA-Ala Resistant3, an Evolutionarily Conserved Target of miR167, Mediates Arabidopsis Root Architecture Changes during High Osmotic Stress. The Plant Cell, 24(9), 3590–3602. doi:10.1105/tpc.112.097006
    Sponsors
    We thank Jason Reed for the generous gifts of arf6-2 and arf8-3 single mutant and arf6-2 arf8-3 double mutant seeds, our laboratory members for discussions, Nagarajan Chandramouli of Rockefeller Proteomics Resource Center for sample preparation relating to free IAA measurement, and Scott Dewell of the Genomics Resource Center for deep sequencing. N.K. was supported by postdoctoral fellowships from the Uehara Memorial Foundation, Swiss National Science Foundation (for Prospective Researchers), and the Japan Society for the Promotion of Science. This work was supported in part by Bayer Crop Science and in part by the Cooperative Research Program for Agricultural Science and Technology Development (PJ906910), Rural Development Administration, Republic of Korea.
    Publisher
    American Society of Plant Biologists (ASPB)
    Journal
    The Plant Cell
    DOI
    10.1105/tpc.112.097006
    PubMed ID
    22960911
    PubMed Central ID
    PMC3480289
    Additional Links
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480289
    ae974a485f413a2113503eed53cd6c53
    10.1105/tpc.112.097006
    Scopus Count
    Collections
    Articles; Bioscience Program; Computational Bioscience Research Center (CBRC)

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