IAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress
MacPherson, Cameron R.
Hannah, Matthew A.
Chuaa, Nam Hai
Permanent link to this recordhttp://hdl.handle.net/10754/562315
MetadataShow full item record
AbstractThe functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles of stress-responsive microRNAs and their targets are not as well understood. Using small RNA deep sequencing and ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) as a new target of miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site and under high osmotic stress miR167a levels decreased, whereas IAR3 mRNA levels increased. IAR3 hydrolyzes an inactive form of auxin (indole-3-acetic acid [IAA]-alanine) and releases bioactive auxin (IAA), a central phytohormone for root development. In contrast with the wild type, iar3 mutants accumulated reduced IAA levels and did not display high osmotic stress-induced root architecture changes. Transgenic plants expressing a cleavage-resistant form of IAR3 mRNA accumulated high levels of IAR3 mRNAs and showed increased lateral root development compared with transgenic plants expressing wild-type IAR3. Expression of an inducible noncoding RNA to sequester miR167a by target mimicry led to an increase in IAR3 mRNA levels, further confirming the inverse relationship between the two partners. Sequence comparison revealed the miR167 target site on IAR3 mRNA is conserved in evolutionarily distant plant species. Finally, we showed that IAR3 is required for drought tolerance. © 2012 American Society of Plant Biologists. All rights reserved.
SponsorsWe thank Jason Reed for the generous gifts of arf6-2 and arf8-3 single mutant and arf6-2 arf8-3 double mutant seeds, our laboratory members for discussions, Nagarajan Chandramouli of Rockefeller Proteomics Resource Center for sample preparation relating to free IAA measurement, and Scott Dewell of the Genomics Resource Center for deep sequencing. N.K. was supported by postdoctoral fellowships from the Uehara Memorial Foundation, Swiss National Science Foundation (for Prospective Researchers), and the Japan Society for the Promotion of Science. This work was supported in part by Bayer Crop Science and in part by the Cooperative Research Program for Agricultural Science and Technology Development (PJ906910), Rural Development Administration, Republic of Korea.
JournalThe Plant Cell
PubMed Central IDPMC3480289
- Hormone crosstalk in wound stress response: wound-inducible amidohydrolases can simultaneously regulate jasmonate and auxin homeostasis in Arabidopsis thaliana.
- Authors: Zhang T, Poudel AN, Jewell JB, Kitaoka N, Staswick P, Matsuura H, Koo AJ
- Issue date: 2016 Mar
- Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by microRNA847 upregulates auxin signaling to modulate cell proliferation and lateral organ growth in Arabidopsis.
- Authors: Wang JJ, Guo HS
- Issue date: 2015 Mar
- MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development.
- Authors: Guo HS, Xie Q, Fei JF, Chua NH
- Issue date: 2005 May
- Cyclic GMP is involved in auxin signalling during Arabidopsis root growth and development.
- Authors: Nan W, Wang X, Yang L, Hu Y, Wei Y, Liang X, Mao L, Bi Y
- Issue date: 2014 Apr
- A family of auxin-conjugate hydrolases that contributes to free indole-3-acetic acid levels during Arabidopsis germination.
- Authors: Rampey RA, LeClere S, Kowalczyk M, Ljung K, Sandberg G, Bartel B
- Issue date: 2004 Jun