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dc.contributor.authorFanucchi, Francesca
dc.contributor.authorAlpi, Emanuele
dc.contributor.authorOlivieri, Stefano
dc.contributor.authorCannistraci, Carlo
dc.contributor.authorBachi, Angela
dc.contributor.authorAlpi, Amedeo
dc.contributor.authorAlessio, Massimo
dc.date.accessioned2015-08-03T09:47:20Z
dc.date.available2015-08-03T09:47:20Z
dc.date.issued2012-06
dc.identifier.issn15709639
dc.identifier.pmid22510494
dc.identifier.doi10.1016/j.bbapap.2012.03.015
dc.identifier.urihttp://hdl.handle.net/10754/562210
dc.description.abstractThis study used 2DE to investigate how Arabidopsis thaliana modulates protein levels in response to freezing stress after sub-lethal exposure at - 10 °C, both in cold-acclimated and in non-acclimated plants. A map was implemented in which 62 spots, corresponding to 44 proteins, were identified. Twenty-two spots were modulated upon treatments, and the corresponding proteins proved to be related to photosynthesis, energy metabolism, and stress response. Proteins demonstrated differences between control and acclimation conditions. Most of the acclimation-responsive proteins were either not further modulated or they were down-modulated by freezing treatment, indicating that the levels reached during acclimation were sufficient to deal with freezing. Anabolic metabolism appeared to be down-regulated in favor of catabolic metabolism. Acclimated plants and plants submitted to freezing after acclimation showed greater reciprocal similarity in protein profiles than either showed when compared both to control plants and to plants frozen without acclimation. The response of non-acclimated plants was aimed at re-modulating photosynthetic apparatus activity, and at increasing the levels of proteins with antioxidant-, molecular chaperone-, or post-transcriptional regulative functions. These changes, even less effective than the acclimation strategy, might allow the injured plastids to minimize the production of non-useful metabolites and might counteract photosynthetic apparatus injuries. © 2012 Elsevier B.V. All rights reserved.
dc.description.sponsorshipMassimo Alessio is supported by MoH RF-FSR-2007-637144 and by AIRC.
dc.publisherElsevier BV
dc.subject2D electrophoresis
dc.subjectArabidopsis thaliana
dc.subjectFreezing stress
dc.subjectMass spectrometry
dc.titleAcclimation increases freezing stress response of Arabidopsis thaliana at proteome level
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics
dc.contributor.institutionDepartment of Crop Plant Biology, University of Pisa, via Mariscoglio 34, 56100 Pisa, Italy
dc.contributor.institutionBiological Mass Spectrometry Unit, San Raffaele Scientific Institute, Milan, Italy
dc.contributor.institutionProteome Biochemistry Unit, San Raffaele Scientific Institute, Milan, Italy
dc.contributor.institutionDepartment of Mechanics, Politecnico di Torino, Turin, Italy
kaust.personCannistraci, Carlo


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