Acclimation increases freezing stress response of Arabidopsis thaliana at proteome level
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Permanent link to this recordhttp://hdl.handle.net/10754/562210
MetadataShow full item record
AbstractThis study used 2DE to investigate how Arabidopsis thaliana modulates protein levels in response to freezing stress after sub-lethal exposure at - 10 °C, both in cold-acclimated and in non-acclimated plants. A map was implemented in which 62 spots, corresponding to 44 proteins, were identified. Twenty-two spots were modulated upon treatments, and the corresponding proteins proved to be related to photosynthesis, energy metabolism, and stress response. Proteins demonstrated differences between control and acclimation conditions. Most of the acclimation-responsive proteins were either not further modulated or they were down-modulated by freezing treatment, indicating that the levels reached during acclimation were sufficient to deal with freezing. Anabolic metabolism appeared to be down-regulated in favor of catabolic metabolism. Acclimated plants and plants submitted to freezing after acclimation showed greater reciprocal similarity in protein profiles than either showed when compared both to control plants and to plants frozen without acclimation. The response of non-acclimated plants was aimed at re-modulating photosynthetic apparatus activity, and at increasing the levels of proteins with antioxidant-, molecular chaperone-, or post-transcriptional regulative functions. These changes, even less effective than the acclimation strategy, might allow the injured plastids to minimize the production of non-useful metabolites and might counteract photosynthetic apparatus injuries. © 2012 Elsevier B.V. All rights reserved.
CitationFanucchi, F., Alpi, E., Olivieri, S., Cannistraci, C. V., Bachi, A., Alpi, A., & Alessio, M. (2012). Acclimation increases freezing stress response of Arabidopsis thaliana at proteome level. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1824(6), 813–825. doi:10.1016/j.bbapap.2012.03.015
SponsorsMassimo Alessio is supported by MoH RF-FSR-2007-637144 and by AIRC.
- Rapid transcriptional and metabolic regulation of the deacclimation process in cold acclimated Arabidopsis thaliana.
- Authors: Pagter M, Alpers J, Erban A, Kopka J, Zuther E, Hincha DK
- Issue date: 2017 Sep 16
- Proteome dynamics of cold-acclimating Rhododendron species contrasting in their freezing tolerance and thermonasty behavior.
- Authors: Die JV, Arora R, Rowland LJ
- Issue date: 2017
- Natural genetic variation in acclimation capacity at sub-zero temperatures after cold acclimation at 4 degrees C in different Arabidopsis thaliana accessions.
- Authors: Le MQ, Engelsberger WR, Hincha DK
- Issue date: 2008 Oct
- Putrescine is involved in Arabidopsis freezing tolerance and cold acclimation by regulating abscisic acid levels in response to low temperature.
- Authors: Cuevas JC, López-Cobollo R, Alcázar R, Zarza X, Koncz C, Altabella T, Salinas J, Tiburcio AF, Ferrando A
- Issue date: 2008 Oct
- Temporal proteomics of Arabidopsis plasma membrane during cold- and de-acclimation.
- Authors: Miki Y, Takahashi D, Kawamura Y, Uemura M
- Issue date: 2019 Apr 15