Arabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water status
Des Marais, David L.
Parsons, Eugene P.
Bangarusamy, Dhinoth Kumar
Juenger, Thomas E.
Bressan, Ray Anthony
Jenks, Matthew A.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/562195
MetadataShow full item record
AbstractMutation of the ECERIFERUM9 (CER9) gene in Arabidopsis (Arabidopsis thaliana) causes elevated amounts of 18-carbon-length cutin monomers and a dramatic shift in the cuticular wax profile (especially on leaves) toward the very-long-chain free fatty acids tetracosanoic acid (C24) and hexacosanoic acid (C26). Relative to the wild type, cer9 mutants exhibit elevated cuticle membrane thickness over epidermal cells and cuticular ledges with increased occlusion of the stomatal pore. The cuticular phenotypes of cer9 are associated with delayed onset of wilting in plants experiencing water deficit, lower transpiration rates, and improved water use efficiency measured as carbon isotope discrimination. The CER9 protein thus encodes a novel determinant of plant drought tolerance-associated traits, one whose deficiency elevates cutin synthesis, redistributes wax composition, and suppresses transpiration. Map-based cloning identified CER9, and sequence analysis predicted that it encodes an E3 ubiquitin ligase homologous to yeast Doa10 (previously shown to target endoplasmic reticulum proteins for proteasomal degradation). To further elucidate CER9 function, the impact of CER9 deficiency on interactions with other genes was examined using double mutant and transcriptome analyses. For both wax and cutin, cer9 showed mostly additive effects with cer6, long-chain acyl-CoA synthetase1 (lacs1), and lacs2 and revealed its role in early steps of both wax and cutin synthetic pathways. Transcriptome analysis revealed that the cer9 mutation affected diverse cellular processes, with primary impact on genes associated with diverse stress responses. The discovery of CER9 lays new groundwork for developing novel cuticle-based strategies for improving the drought tolerance and water use efficiency of crop plants. © 2012 American Society of Plant Biologists. All Rights Reserved.
SponsorsThis work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grant to O.R.), the National Science Foundation (grant no. DEB-0618347 to T.J.), and the U.S. Department of Agriculture (National Institute of Food and Agriculture Biomass Research and Development Initiative grant to M.A.J.).
PubMed Central IDPMC3387718
- Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis.
- Authors: Weng H, Molina I, Shockey J, Browse J
- Issue date: 2010 Apr
- Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis.
- Authors: Lü S, Song T, Kosma DK, Parsons EP, Rowland O, Jenks MA
- Issue date: 2009 Aug
- Histone H2B monoubiquitination is involved in the regulation of cutin and wax composition in Arabidopsis thaliana.
- Authors: Ménard R, Verdier G, Ors M, Erhardt M, Beisson F, Shen WH
- Issue date: 2014 Feb
- Cuticle ultrastructure, cuticular lipid composition, and gene expression in hypoxia-stressed Arabidopsis stems and leaves.
- Authors: Kim H, Choi D, Suh MC
- Issue date: 2017 Jun
- The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers.
- Authors: Yang X, Zhao H, Kosma DK, Tomasi P, Dyer JM, Li R, Liu X, Wang Z, Parsons EP, Jenks MA, Lü S
- Issue date: 2017 Feb