Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope
Permanent link to this recordhttp://hdl.handle.net/10754/562147
MetadataShow full item record
AbstractThe elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. © 2011 Elsevier Ltd.
CitationZhou, Z. L., Ngan, A. H. W., Tang, B., & Wang, A. X. (2012). Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope. Journal of the Mechanical Behavior of Biomedical Materials, 8, 134–142. doi:10.1016/j.jmbbm.2011.11.010
SponsorsThe work done in this article was supported by a grant from the University Grants Committee of the Hong Kong Special Administration Region, PR China (Project No. SEG-HKU06).
- Surface detection errors cause overestimation of the modulus in nanoindentation on soft materials.
- Authors: Kaufman JD, Klapperich CM
- Issue date: 2009 Aug
- Measuring viscoelasticity of soft samples using atomic force microscopy.
- Authors: Tripathy S, Berger EJ
- Issue date: 2009 Sep
- Quantitative measurement of indentation hardness and modulus of compliant materials by atomic force microscopy.
- Authors: Passeri D, Bettucci A, Biagioni A, Rossi M, Alippi A, Lucci M, Davoli I, Berezina S
- Issue date: 2008 Jun
- Spatially resolved frequency-dependent elasticity measured with pulsed force microscopy and nanoindentation.
- Authors: Sweers KK, van der Werf KO, Bennink ML, Subramaniam V
- Issue date: 2012 Mar 21
- Nanomechanics of biocompatible TiO(2) nanotubes by Interfacial Force Microscopy (IFM).
- Authors: Crawford GA, Chawla N, Houston JE
- Issue date: 2009 Dec