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    17β-estradiol rapidly activates calcium release from intracellular stores via the GPR30 pathway and MAPK phosphorylation in osteocyte-like MLO-Y4 cells

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    Type
    Article
    Authors
    Ren, Jian
    Wu, Junhua
    KAUST Department
    Imaging and Characterization Core Lab
    Advanced Nanofabrication, Imaging and Characterization Core Lab
    Core Labs
    Date
    2012-03-06
    Online Publication Date
    2012-03-06
    Print Publication Date
    2012-05
    Permanent link to this record
    http://hdl.handle.net/10754/562124
    
    Metadata
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    Abstract
    Estrogen regulates critical cellular functions, and its deficiency initiates bone turnover and the development of bone mass loss in menopausal females. Recent studies have demonstrated that 17β-estradiol (E 2) induces rapid non-genomic responses that activate downstream signaling molecules, thus providing a new perspective to understand the relationship between estrogen and bone metabolism. In this study, we investigated rapid estrogen responses, including calcium release and MAPK phosphorylation, in osteocyte-like MLO-Y4 cells. E 2 elevated [Ca 2+] i and increased Ca 2+ oscillation frequency in a dose-dependent manner. Immunolabeling confirmed the expression of three estrogen receptors (ERα, ERβ, and G protein-coupled receptor 30 [GPR30]) in MLO-Y4 cells and localized GPR30 predominantly to the plasma membrane. E 2 mobilized calcium from intracellular stores, and the use of selective agonist(s) for each ER showed that this was mediated mainly through the GPR30 pathway. MAPK phosphorylation increased in a biphasic manner, with peaks occurring after 7 and 60 min. GPR30 and classical ERs showed different temporal effects on MAPK phosphorylation and contributed to MAPK phosphorylation sequentially. ICI182,780 inhibited E 2 activation of MAPK at 7 min, while the GPR30 agonist G-1 and antagonist G-15 failed to affect MAPK phosphorylation levels. G-1-mediated MAPK phosphorylation at 60 min was prevented by prior depletion of calcium stores. Our data suggest that E 2 induces the non-genomic responses Ca 2+ release and MAPK phosphorylation to regulate osteocyte function and indicate that multiple receptors mediate rapid E 2 responses. © 2012 Springer Science+Business Media, LLC.
    Citation
    Ren, J., & Wu, J. H. (2012). 17β-Estradiol Rapidly Activates Calcium Release from Intracellular Stores via the GPR30 Pathway and MAPK Phosphorylation in Osteocyte-Like MLO-Y4 Cells. Calcified Tissue International, 90(5), 411–419. doi:10.1007/s00223-012-9581-x
    Sponsors
    We are indebted to Dr. Lynda Bonewald for her kind gift of MLO Y4 cells and thank Ms. Jennifer Rosser for help in arranging the transportation and logistics. This work was supported by the Nature Science Foundation of Shanghai, China (Grant 11ZR1440700).
    Publisher
    Springer Nature
    Journal
    Calcified Tissue International
    DOI
    10.1007/s00223-012-9581-x
    PubMed ID
    22392527
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00223-012-9581-x
    Scopus Count
    Collections
    Articles; Imaging and Characterization Core Lab

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