• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Ornelas-Megiatto, Cátia
    Wich, Peter R.
    Frechet, Jean cc
    KAUST Department
    Chemical Science Program
    Office of the VP
    Physical Science and Engineering (PSE) Division
    Date
    2012-01-19
    Online Publication Date
    2012-01-19
    Print Publication Date
    2012-02
    Permanent link to this record
    http://hdl.handle.net/10754/562078
    
    Metadata
    Show full item record
    Abstract
    A water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. © 2012 American Chemical Society.
    Sponsors
    This project has been funded in part with Federal funds from the National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN268201000043C, and in part through the Frechet "various donors" fund for the support of research in new materials. We thank Ann Fischer and Michelle Yasukawa for help with cell culture and Dr. Peter Friebe and Dr. Eva Harris for assistance with and the use of their luminescence plate reader. P.R.W. gratefully acknowledges the Alexander von Humboldt Foundation (AvH) for partial funding.
    Publisher
    American Chemical Society (ACS)
    Journal
    Journal of the American Chemical Society
    DOI
    10.1021/ja207366k
    PubMed ID
    22239619
    ae974a485f413a2113503eed53cd6c53
    10.1021/ja207366k
    Scopus Count
    Collections
    Articles; Physical Science and Engineering (PSE) Division; Chemical Science Program

    entitlement

    Related articles

    • Effect of multifold charge groups and imidazole-4-carboxaldehyde on physicochemical characteristics and transfection of cationic polyphosphazenes/DNA complexes.
    • Authors: Yang Y, Zhang Z, Chen L, Li Y
    • Issue date: 2010 May 10
    • Polymer nanocarrier system for endosome escape and timed release of siRNA with complete gene silencing and cell death in cancer cells.
    • Authors: Gu W, Jia Z, Truong NP, Prasadam I, Xiao Y, Monteiro MJ
    • Issue date: 2013 Oct 14
    • Synthesis and characterization of a novel arginine-grafted dendritic block copolymer for gene delivery and study of its cellular uptake pathway leading to transfection.
    • Authors: Kim TI, Baek JU, Yoon JK, Choi JS, Kim K, Park JS
    • Issue date: 2007 Mar-Apr
    • Polyspermine imidazole-4,5-imine, a chemically dynamic and biologically responsive carrier system for intracellular delivery of siRNA.
    • Authors: Duan S, Yuan W, Wu F, Jin T
    • Issue date: 2012 Aug 6
    • Simple modifications of branched PEI lead to highly efficient siRNA carriers with low toxicity.
    • Authors: Zintchenko A, Philipp A, Dehshahri A, Wagner E
    • Issue date: 2008 Jul
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.