The glossyhead1 allele of acc1 reveals a principal role for multidomain acetyl-coenzyme a carboxylase in the biosynthesis of cuticular waxes by Arabidopsis
Type
ArticleAuthors
Lu, ShiyouZhao, Huayan
Parsons, Eugene P.
Xu, Changcheng
Kosma, Dylan K.
Xu, Xiaojing
Chao, Daiyin
Lohrey, Gregory T.
Bangarusamy, Dhinoth Kumar
Wang, Guangchao
Bressan, Ray Anthony
Jenks, Matthew A.
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Core Lab
Bioscience Program
Center for Desert Agriculture
Core Labs
Imaging and Characterization Core Lab
Date
2011-09-23Online Publication Date
2011-09-23Print Publication Date
2011-11-01Permanent link to this record
http://hdl.handle.net/10754/561878
Metadata
Show full item recordAbstract
A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gene was identified as a new allele of Acetyl-Coenzyme A Carboxylase1 (ACC1), a gene encoding the main enzyme in cytosolic malonyl-coenzyme A synthesis. This, to our knowledge, is the first mutant allele of ACC1 that does not cause lethality at the seed or early germination stage, allowing for the first time a detailed analysis of ACC1 function in mature tissues. Broad lipid profiling of mature gsd1 organs revealed a primary role for ACC1 in the biosynthesis of the very-long-chain fatty acids (C 20:0 or longer) associated with cuticular waxes and triacylglycerols. Unexpectedly, transcriptome analysis revealed that gsd1 has limited impact on any lipid metabolic networks but instead has a large effect on environmental stress-responsive pathways, especially senescence and ethylene synthesis determinants, indicating a possible role for the cytosolic malonyl-coenzyme A-derived lipids in stress response signaling. © 2011 American Society of Plant Biologists. All Rights Reserved.Citation
Lü, S., Zhao, H., Parsons, E. P., Xu, C., Kosma, D. K., Xu, X., … Jenks, M. A. (2011). The glossyhead1 Allele of ACC1 Reveals a Principal Role for Multidomain Acetyl-Coenzyme A Carboxylase in the Biosynthesis of Cuticular Waxes by Arabidopsis. Plant Physiology, 157(3), 1079–1092. doi:10.1104/pp.111.185132Sponsors
We are grateful to Dr. Masao Tasaka (Nara Institute of Science and Technology) for providing emb22 and acc1-3 seeds. We also thank Debra Sherman and Chia-Ping Huang of the Purdue University Electron Microscopy Center for support.Journal
PLANT PHYSIOLOGYPubMed ID
21949210PubMed Central ID
PMC3252135Additional Links
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252135ae974a485f413a2113503eed53cd6c53
10.1104/pp.111.185132
Scopus Count
Related articles
- Arabidopsis 3-ketoacyl-coenzyme a synthase9 is involved in the synthesis of tetracosanoic acids as precursors of cuticular waxes, suberins, sphingolipids, and phospholipids.
- Authors: Kim J, Jung JH, Lee SB, Go YS, Kim HJ, Cahoon R, Markham JE, Cahoon EB, Suh MC
- Issue date: 2013 Jun
- Arabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water status.
- Authors: Lü S, Zhao H, Des Marais DL, Parsons EP, Wen X, Xu X, Bangarusamy DK, Wang G, Rowland O, Juenger T, Bressan RA, Jenks MA
- Issue date: 2012 Jul
- gurke and pasticcino3 mutants affected in embryo development are impaired in acetyl-CoA carboxylase.
- Authors: Baud S, Bellec Y, Miquel M, Bellini C, Caboche M, Lepiniec L, Faure JD, Rochat C
- Issue date: 2004 May
- The sensitive to freezing3 mutation of Arabidopsis thaliana is a cold-sensitive allele of homomeric acetyl-CoA carboxylase that results in cold-induced cuticle deficiencies.
- Authors: Amid A, Lytovchenko A, Fernie AR, Warren G, Thorlby GJ
- Issue date: 2012 Sep
- Reverse-genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-coenzyme A carboxylase in Arabidopsis indicates a unidirectional functional redundancy.
- Authors: Li X, Ilarslan H, Brachova L, Qian HR, Li L, Che P, Wurtele ES, Nikolau BJ
- Issue date: 2011 Jan