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    Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

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    Type
    Article
    Authors
    Ramadan, Ahmed M Ali
    Eissa, Hala F.
    El-Domyati, Fotouh M.
    Saleh, Osama Mesilhy
    Ibrahim, Nasser E.
    Salama, M. I.
    Mahfouz, Magdy M. cc
    Bahieldin, Ahmed M.
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Laboratory for Genome Engineering
    Plant Science
    Plant Science Program
    Date
    2011-06-26
    Online Publication Date
    2011-06-26
    Print Publication Date
    2011-12
    Permanent link to this record
    http://hdl.handle.net/10754/561804
    
    Metadata
    Show full item record
    Abstract
    The uidA gene, encoding for β-glucuronidase (GUS), is the most frequently used reporter gene in plants. As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively. In wheat, there are numerous reports of failure in detecting GUS enzyme activity in tissues of transgenic plants, while other reports have suggested presence of β-glucuronidase inhibitor(s) in wheat tissues. In the present study, we show that the β-glucuronidase enzyme activity is not only tissue-specific but also genotype-dependent. Our data demonstrate that the glucuronic acid could be the candidate inhibitor for β-glucuronidase enzyme activity in wheat leaves and roots. It should be noted that the assays to detect β-glucuronidase enzyme activity in wheat should be interpreted carefully. Based on the data of our present study, we recommend studying the chemical pathways, the unintended effects and the possible loss-of-function of any candidate transgene prior to transformation experiments. © 2011 Springer Science+Business Media B.V.
    Citation
    Ramadan, A. M., Eissa, H. F., El-Domyati, F. M., Saleh, O. M., Ibrahim, N. E., Salama, M., … Bahieldin, A. (2011). Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat. Plant Cell, Tissue and Organ Culture (PCTOC), 107(3), 373–381. doi:10.1007/s11240-011-9988-5
    Publisher
    Springer Nature
    Journal
    Plant Cell, Tissue and Organ Culture (PCTOC)
    DOI
    10.1007/s11240-011-9988-5
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11240-011-9988-5
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

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