Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat
Type
ArticleAuthors
Ramadan, Ahmed M AliEissa, Hala F.
El-Domyati, Fotouh M.
Saleh, Osama Mesilhy
Ibrahim, Nasser E.
Salama, M. I.
Mahfouz, Magdy M.

Bahieldin, Ahmed M.
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
Laboratory for Genome Engineering
Plant Science
Plant Science Program
Date
2011-06-26Online Publication Date
2011-06-26Print Publication Date
2011-12Permanent link to this record
http://hdl.handle.net/10754/561804
Metadata
Show full item recordAbstract
The uidA gene, encoding for β-glucuronidase (GUS), is the most frequently used reporter gene in plants. As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively. In wheat, there are numerous reports of failure in detecting GUS enzyme activity in tissues of transgenic plants, while other reports have suggested presence of β-glucuronidase inhibitor(s) in wheat tissues. In the present study, we show that the β-glucuronidase enzyme activity is not only tissue-specific but also genotype-dependent. Our data demonstrate that the glucuronic acid could be the candidate inhibitor for β-glucuronidase enzyme activity in wheat leaves and roots. It should be noted that the assays to detect β-glucuronidase enzyme activity in wheat should be interpreted carefully. Based on the data of our present study, we recommend studying the chemical pathways, the unintended effects and the possible loss-of-function of any candidate transgene prior to transformation experiments. © 2011 Springer Science+Business Media B.V.Citation
Ramadan, A. M., Eissa, H. F., El-Domyati, F. M., Saleh, O. M., Ibrahim, N. E., Salama, M., … Bahieldin, A. (2011). Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat. Plant Cell, Tissue and Organ Culture (PCTOC), 107(3), 373–381. doi:10.1007/s11240-011-9988-5Publisher
Springer Natureae974a485f413a2113503eed53cd6c53
10.1007/s11240-011-9988-5