The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds
AuthorsGerhardt, Lutz Christian
Widdows, Kate L.
Erol, Melek M.
Burch, Charles W.
Sanz-Herrera, José A.
Roqan, Iman S.
Gabe, Simon M.
Ansari, Tahera I.
Boccaccini, Aldo R.
KAUST DepartmentPhysical Sciences and Engineering (PSE) Division
Materials Science and Engineering Program
Semiconductor and Material Spectroscopy (SMS) Laboratory
MetadataShow full item record
AbstractThe angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.
SponsorsThe authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.
- Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds.
- Authors: Gerhardt LC, Widdows KL, Erol MM, Nandakumar A, Roqan IS, Ansari T, Boccaccini AR
- Issue date: 2013 Mar
- Hypoxia-mimicking bioactive glass/collagen glycosaminoglycan composite scaffolds to enhance angiogenesis and bone repair.
- Authors: Quinlan E, Partap S, Azevedo MM, Jell G, Stevens MM, O'Brien FJ
- Issue date: 2015 Jun
- Biocompatibility and osteogenesis of biomimetic Bioglass-Collagen-Phosphatidylserine composite scaffolds for bone tissue engineering.
- Authors: Xu C, Su P, Chen X, Meng Y, Yu W, Xiang AP, Wang Y
- Issue date: 2011 Feb
- PDLLA scaffolds with Cu- and Zn-doped bioactive glasses having multifunctional properties for bone regeneration.
- Authors: Bejarano J, Detsch R, Boccaccini AR, Palza H
- Issue date: 2017 Mar
- Three-dimensional zinc incorporated borosilicate bioactive glass scaffolds for rodent critical-sized calvarial defects repair and regeneration.
- Authors: Wang H, Zhao S, Xiao W, Cui X, Huang W, Rahaman MN, Zhang C, Wang D
- Issue date: 2015 Jun 1