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    Development of SCAR marker specific to non-toxic Jatropha curcas L. and designing a novel multiplexing PCR along with nrDNA ITS primers to circumvent the false negative detection

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    Type
    Article
    Authors
    Mastan, Shaik G.
    Pamidimarri, D. V N N Sudheer
    Rahman, Hifzur
    Reddy, Muppala P.
    Chikara, Jitendra
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Center for Desert Agriculture
    Date
    2011-05-10
    Online Publication Date
    2011-05-10
    Print Publication Date
    2012-01
    Permanent link to this record
    http://hdl.handle.net/10754/561774
    
    Metadata
    Show full item record
    Abstract
    Jatropha curcas L., a multipurpose shrub, has acquired significant economic importance for its seed oil which can be converted to biodiesel an emerging alternative to petro-diesel. In addition to the commercial value, it is also having medicinal and even high nutritional value to use as animal fodder which is limited due to the toxicity. Development of molecular marker will enable to differentiate non-toxic from toxic variety of J. curcas in a mixed population and also for quality control since the toxic components of J. curcas has deleterious effect on animals. In the present study, the efforts were made to generate the specific SCAR marker for toxic and/or non-toxic J. curcas from RAPD markers. Among the markers specific for toxic and non-toxic varieties, four were selected, purified, cloned, sequenced, and designed primers out of which one set of primers NT-JC/SCAR I/OPQ15-F and R could able to discriminate the non-toxic with toxic Jatropha by giving expected 430 bp size amplification in non-toxic variety. Furthermore, novel multiplex PCR was designed using the nrDNA ITS primers to overcome the false negatives. Present work also demonstrates utility of the conserved regions of nrDNA coding genes in ruling out the artifacts in PCR-like false negatives frequently occur in SCAR due to various reasons. The specific SCAR markers generated in the present investigation will help to distinguish non-toxic from toxic varieties of J. curcas or vice versa, and isolated marker along with designed multiplex protocol has applications in quality control for selective cultivation of non-toxic variety and will also assist in breeding and molecular mapping studies. © 2011 Springer Science+Business Media, LLC.
    Citation
    Mastan, S. G., Sudheer, P. D. V. N., Rahman, H., Reddy, M. P., & Chikara, J. (2011). Development of SCAR Marker Specific to Non-Toxic Jatropha curcas L. and Designing a Novel Multiplexing PCR Along with nrDNA ITS Primers to Circumvent the False Negative Detection. Molecular Biotechnology, 50(1), 57–61. doi:10.1007/s12033-011-9415-5
    Sponsors
    We are thankful to Council for Scientific and Industrial Research, New Delhi, India, for its financial support and for research associate fellowship. We would like to thank Professor B. N. Tiwari, Head, School of biotechnology, for his kind support and encouragement.
    Publisher
    Springer Nature
    Journal
    Molecular Biotechnology
    DOI
    10.1007/s12033-011-9415-5
    PubMed ID
    21556845
    ae974a485f413a2113503eed53cd6c53
    10.1007/s12033-011-9415-5
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Center for Desert Agriculture

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