The plant cuticle is required for osmotic stress regulation of abscisic acid biosynthesis and osmotic stress tolerance in Arabidopsis
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Plant Stress Genomics Research Lab
Online Publication Date2011-05-24
Print Publication Date2011-05
Permanent link to this recordhttp://hdl.handle.net/10754/561772
MetadataShow full item record
AbstractOsmotic stress activates the biosynthesis of abscisic acid (ABA). One major step in ABA biosynthesis is the carotenoid cleavage catalyzed by a 9-cis epoxycarotenoid dioxygenase (NCED). To understand the mechanism for osmotic stress activation of ABA biosynthesis, we screened for Arabidopsis thaliana mutants that failed to induce the NCED3 genee xpression in response to osmotic stress treatments. The ced1 (for 9-cis epoxycarotenoid dioxy genase defective 1) mutant isolated in this study showed markedly reduced expression of NCED3 in response to osmotic stress (polyethylene glycol)treatments compared with the wild type. Other ABA biosynthesis genes are also greatly reduced in ced1 under osmotic stress. ced1 mutant plants are very sensitive to even mild osmotic stress. Map-based cloning revealed unexpectedly thatCED1 encodes a putative a/b hydrolase domain-containing protein and is allelic to the BODYGUARD gene that was recently shown to be essential for cuticle biogenesis. Further studies discovered that other cut in biosynthesis mutants are also impaired in osmotic stress induction of ABA biosynthesis genes and are sensitive to osmotic stress. Our work demonstrates that the cuticle functions not merely as a physical barrier to minimize water loss but also mediates osmotic stress signaling and tolerance by regulating ABA biosynthesis and signaling. © 2011 American Society of Plant Biologists. All rights reserved.
SponsorsWe thank Paul E. Verslues for help with the initial phase of the work, Iida Kei for help with analyzing the microarray data, and Rebecca Stevenson for technical assistance. This work was supported by National Institutes of Health Grants R01GM070795 and R01GM059138 to J.-K.Z. and by National Science Foundation Grant IOS0919745 to J.Z.
JournalThe Plant Cell
PubMed Central IDPMC3123942
- The Arabidopsis Vacuolar Sorting Receptor1 is required for osmotic stress-induced abscisic acid biosynthesis.
- Authors: Wang ZY, Gehring C, Zhu J, Li FM, Zhu JK, Xiong L
- Issue date: 2015 Jan
- <i>Arabidopsis thaliana</i> NGATHA1 transcription factor induces ABA biosynthesis by activating <i>NCED3</i> gene during dehydration stress.
- Authors: Sato H, Takasaki H, Takahashi F, Suzuki T, Iuchi S, Mitsuda N, Ohme-Takagi M, Ikeda M, Seo M, Yamaguchi-Shinozaki K, Shinozaki K
- Issue date: 2018 Nov 20
- Arabidopsis lenc1 mutant displays reduced ABA accumulation by low AtNCED3 expression under osmotic stress.
- Authors: Woo DH, Park HY, Kang IS, Lee SY, Moon BY, Lee CB, Moon YH
- Issue date: 2011 Jan 15
- MhNCED3, a gene encoding 9-cis-epoxycarotenoid dioxygenase in Malus hupehensis Rehd., enhances plant tolerance to Cl- stress by reducing Cl- accumulation.
- Authors: Zhang WW, Yang HQ, You SZ, Fan SL, Ran K
- Issue date: 2015 Apr
- Epoxycarotenoid cleavage by NCED5 fine-tunes ABA accumulation and affects seed dormancy and drought tolerance with other NCED family members.
- Authors: Frey A, Effroy D, Lefebvre V, Seo M, Perreau F, Berger A, Sechet J, To A, North HM, Marion-Poll A
- Issue date: 2012 May