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dc.contributor.authorWang, Zhijuan
dc.contributor.authorZhang, Juan
dc.contributor.authorChen, Peng
dc.contributor.authorZhou, Xiaozhu
dc.contributor.authorYang, Yanli
dc.contributor.authorWu, Shixin
dc.contributor.authorNiu, Li
dc.contributor.authorHan, Yu
dc.contributor.authorWang, Lianhui
dc.contributor.authorChen, Peng
dc.contributor.authorBoey, Freddy
dc.contributor.authorZhang, Qichun
dc.contributor.authorLiedberg, Bo Gunnar
dc.contributor.authorZhang, Hua
dc.date.accessioned2015-08-03T09:04:04Z
dc.date.available2015-08-03T09:04:04Z
dc.date.issued2011-05
dc.identifier.citationWang, Z., Zhang, J., Chen, P., Zhou, X., Yang, Y., Wu, S., … Zhang, H. (2011). Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes. Biosensors and Bioelectronics, 26(9), 3881–3886. doi:10.1016/j.bios.2011.03.002
dc.identifier.issn09565663
dc.identifier.pmid21458255
dc.identifier.doi10.1016/j.bios.2011.03.002
dc.identifier.urihttp://hdl.handle.net/10754/561763
dc.description.abstractReduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.
dc.description.sponsorshipThis work was supported by the AcRF Tier 2 (ARC 10/10, no. MOE2010-T2-1-060) from MOE, POC (S08/1-82563404) from EDB, CRP (NRF-CRP2-2007-01) from NRF, an A*STAR SERC Grant (no. 092 101 0064) from A*STAR,New Initiative Fund FY 2010 (M58120031) from NTU, and the Centre for Biomimetic Sensor Science at NTU in Singapore. P.C. thanks the financial support from A*STAR SERC (grant no. 072 101 0020) in Singapore. B.L. also acknowledges support from the Swedish Research Council and the NanoSence program the Foundation for Strategic Research.
dc.publisherElsevier BV
dc.subjectDNA
dc.subjectGraphene
dc.subjectImpedance spectroscopy
dc.subjectLabel-free detection
dc.subjectMethicillin-resistant Staphylococcus aureus
dc.subjectReduced graphene oxide
dc.titleLabel-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes
dc.typeArticle
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Center
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentChemical Science Program
dc.contributor.departmentNanostructured Functional Materials (NFM) laboratory
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalBiosensors and Bioelectronics
dc.contributor.institutionSchool of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, SGP 639798, Singapore
dc.contributor.institutionCenter for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, SGP 637553, Singapore
dc.contributor.institutionState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
dc.contributor.institutionLaboratory of Advanced Materials, Fudan University, Shanghai 200438, China
dc.contributor.institutionSchool of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, SGP 637457, Singapore
kaust.personHan, Yu


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