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dc.contributor.authorArsenescu, Violeta
dc.contributor.authorNarasimhan, Meena L.
dc.contributor.authorHalide, Tuna
dc.contributor.authorBressan, Ray Anthony
dc.contributor.authorBarisione, Chiara
dc.contributor.authorCohen, Donald A.
dc.contributor.authorDe Villiers, Willem Js S
dc.contributor.authorArsenescu, Razvan I.
dc.date.accessioned2015-08-03T09:03:47Z
dc.date.available2015-08-03T09:03:47Z
dc.date.issued2011-04-11
dc.identifier.issn01632116
dc.identifier.pmid21479819
dc.identifier.doi10.1007/s10620-011-1692-0
dc.identifier.urihttp://hdl.handle.net/10754/561751
dc.description.abstractBackground: Hypoadiponectinemia has been associated with states of chronic inflammation in humans. Mesenteric fat hypertrophy and low adiponectin have been described in patients with Crohn's disease. We investigated whether adiponectin and the plant-derived homolog, osmotin, are beneficial in a murine model of colitis. Methods: C57BL/6 mice were injected (i.v.) with an adenoviral construct encoding the full-length murine adiponectin gene (AN+DSS) or a reporter-LacZ (Ctr and V+DSS groups) prior to DSS colitis protocol. In another experiment, mice with DSS colitis received either osmotin (Osm+DSS) or saline (DSS) via osmotic pumps. Disease progression and severity were evaluated using body weight, stool consistency, rectal bleeding, colon lengths, and histology. In vitro experiments were carried out in bone marrow-derived dendritic cells. Results: Mice overexpressing adiponectin had lower expression of proinflammatory cytokines (TNF, IL-1β), adipokines (angiotensin, osteopontin), and cellular stress and apoptosis markers. These mice had higher levels of IL-10, alternative macrophage marker, arginase 1, and leukoprotease inhibitor. The plant adiponectin homolog osmotin similarly improved colitis outcome and induced robust IL-10 secretion. LPS induced a state of adiponectin resistance in dendritic cells that was reversed by treatment with PPARγ agonist and retinoic acid. Conclusion: Adiponectin exerted protective effects during murine DSS colitis. It had a broad activity that encompassed cytokines, chemotactic factors as well as processes that assure cell viability during stressful conditions. Reducing adiponectin resistance or using plant-derived adiponectin homologs may become therapeutic options in inflammatory bowel disease. © 2011 Springer Science+Business Media, LLC.
dc.description.sponsorshipThis work was supported by grants from Broad Medical Foundation, UCB Inc., Kentucky Science & Engineering Foundation (RA), and from NIH-DK07778-07 (VA). There are no competing financial interests to declare.
dc.publisherSpringer Science + Business Media
dc.subjectAdiponectin
dc.subjectDendritic cells
dc.subjectInflammatory bowel disease
dc.subjectMammalian adiponectin homolog
dc.subjectPPARγ agonists
dc.titleAdiponectin and plant-derived mammalian adiponectin homolog exert a protective effect in murine colitis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalDigestive Diseases and Sciences
dc.contributor.institutionDivision of Digestive Diseases and Nutrition, Microbiology, Immunology and Molecular Genetics, University of Kentucky, Medical Center, 800 Rose Street, Lexington, KY 40536, United States
dc.contributor.institutionGraduate Center for Nutritional Sciences, University of Kentucky, Lexington, KY 40536, United States
dc.contributor.institutionMicrobiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40536, United States
dc.contributor.institutionResearch Center of Cardiovascular Biology, University of Genoa, Liguria 16126, Italy
dc.contributor.institutionDivision of Applied Sciences, WCU Program, Gyeongsang National University, Jinju 660-701, South Korea
dc.contributor.institutionDepartments of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
kaust.personBressan, Ray Anthony
kaust.personNarasimhan, Meena L.


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