Plant natriuretic peptides are apoplastic and paracrine stress response molecules
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Molecular Signalling Group
Date
2011-04-07Online Publication Date
2011-04-07Print Publication Date
2011-05Permanent link to this record
http://hdl.handle.net/10754/561750
Metadata
Show full item recordAbstract
Higher plants contain biologically active proteins that are recognized by antibodies against human atrial natriuretic peptide (ANP). We identified and isolated two Arabidopsis thaliana immunoreactive plant natriuretic peptide (PNP)-encoding genes, AtPNP-A and AtPNP-B, which are distantly related members of the expansin superfamily and have a role in the regulation of homeostasis in abiotic and biotic stresses, and have shown that AtPNP-A modulates the effects of ABA on stomata. Arabidopsis PNP (PNP-A) is mainly expressed in leaf mesophyll cells, and in protoplast assays we demonstrate that it is secreted using AtPNP-A:green fluorescent protein (GFP) reporter constructs and flow cytometry. Transient reporter assays provide evidence that AtPNP-A expression is enhanced by heat, osmotica and salt, and that AtPNP-A itself can enhance its own expression, thereby generating a response signature diagnostic for paracrine action and potentially also autocrine effects. Expression of native AtPNP-A is enhanced by osmotica and transiently by salt. Although AtPNP-A expression is induced by salt and osmotica, ABA does not significantly modulate AtPNP-A levels nor does recombinant AtPNP-A affect reporter expression of the ABA-responsive RD29A gene. Together, these results provide experimental evidence that AtPNP-A is stress responsive, secreted into the apoplastic space and can enhance its own expression. Furthermore, our findings support the idea that AtPNP-A, together with ABA, is an important component in complex plant stress responses and that, much like in animals, peptide signaling molecules can create diverse and modular signals essential for growth, development and defense under rapidly changing environmental conditions. © 2011 The Author.Citation
Wang, Y. H., Gehring, C., & Irving, H. R. (2011). Plant Natriuretic Peptides are Apoplastic and Paracrine Stress Response Molecules. Plant and Cell Physiology, 52(5), 837–850. doi:10.1093/pcp/pcr036Sponsors
This work was supported by the Australian Research Council [Discovery project funding scheme (DP0557561, DP0878194)]; Australian Postgraduate Award [to Y.H.W.].Publisher
Oxford University Press (OUP)Journal
Plant and Cell PhysiologyPubMed ID
21478192ae974a485f413a2113503eed53cd6c53
10.1093/pcp/pcr036
Scopus Count
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