KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Plant Science Program
Permanent link to this recordhttp://hdl.handle.net/10754/561749
MetadataShow full item record
AbstractSite-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditis elegans, mammalian and plant cells. The genomic DSBs can be generated at predefined and user-selected loci and repaired by either the non-homologous end joining (NHEJ) or homology dependent repair (HDR). Thus, TALENs can be used to achieve site-specific gene addition, stacking, deletion or inactivation. TALE-based genome engineering tools should be powerful to develop new agricultural biotechnology approaches for crop improvement. Here, we discuss the recent research and the potential applications of TALENs to accelerate the generation of genomic variants through targeted mutagenesis and to produce a non-transgenic GM crops with the desired phenotype.
PublisherInforma UK Limited
- Towards targeted mutagenesis and gene replacement in plants.
- Authors: Tzfira T, White C
- Issue date: 2005 Dec
- Genome editing for crop improvement: Challenges and opportunities.
- Authors: Abdallah NA, Prakash CS, McHughen AG
- Issue date: 2015
- TALENs: customizable molecular DNA scissors for genome engineering of plants.
- Authors: Chen K, Gao C
- Issue date: 2013 Jun 20
- Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives.
- Authors: Mahfouz MM, Piatek A, Stewart CN Jr
- Issue date: 2014 Oct
- Precise Genome Modification via Sequence-Specific Nucleases-Mediated Gene Targeting for Crop Improvement.
- Authors: Sun Y, Li J, Xia L
- Issue date: 2016