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    TALE nucleases and next generation GM crops.

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    Type
    Article
    Authors
    Mahfouz, Magdy M. cc
    Li, Lixin
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Laboratory for Genome Engineering
    Plant Science
    Plant Science Program
    Date
    2011-04-01
    Permanent link to this record
    http://hdl.handle.net/10754/561749
    
    Metadata
    Show full item record
    Abstract
    Site-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditis elegans, mammalian and plant cells. The genomic DSBs can be generated at predefined and user-selected loci and repaired by either the non-homologous end joining (NHEJ) or homology dependent repair (HDR). Thus, TALENs can be used to achieve site-specific gene addition, stacking, deletion or inactivation. TALE-based genome engineering tools should be powerful to develop new agricultural biotechnology approaches for crop improvement. Here, we discuss the recent research and the potential applications of TALENs to accelerate the generation of genomic variants through targeted mutagenesis and to produce a non-transgenic GM crops with the desired phenotype.
    Citation
    Mahfouz, M. M., & Li, L. (2011). TALE nucleases and next generation GM crops. GM Crops, 2(2), 99–103. doi:10.4161/gmcr.2.2.17254
    Publisher
    Informa UK Limited
    Journal
    GM Crops
    DOI
    10.4161/gmcr.2.2.17254
    PubMed ID
    21865862
    ae974a485f413a2113503eed53cd6c53
    10.4161/gmcr.2.2.17254
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

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