Type
ArticleAuthors
Mahfouz, Magdy M.
Li, Lixin
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
Laboratory for Genome Engineering
Plant Science
Plant Science Program
Date
2011-04-01Permanent link to this record
http://hdl.handle.net/10754/561749
Metadata
Show full item recordAbstract
Site-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditis elegans, mammalian and plant cells. The genomic DSBs can be generated at predefined and user-selected loci and repaired by either the non-homologous end joining (NHEJ) or homology dependent repair (HDR). Thus, TALENs can be used to achieve site-specific gene addition, stacking, deletion or inactivation. TALE-based genome engineering tools should be powerful to develop new agricultural biotechnology approaches for crop improvement. Here, we discuss the recent research and the potential applications of TALENs to accelerate the generation of genomic variants through targeted mutagenesis and to produce a non-transgenic GM crops with the desired phenotype.Citation
Mahfouz, M. M., & Li, L. (2011). TALE nucleases and next generation GM crops. GM Crops, 2(2), 99–103. doi:10.4161/gmcr.2.2.17254Publisher
Informa UK LimitedJournal
GM CropsPubMed ID
21865862ae974a485f413a2113503eed53cd6c53
10.4161/gmcr.2.2.17254
Scopus Count
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