NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis
Type
ArticleAuthors
Choi, WonkyunBaek, Dongwon
Oh, Dongha
Park, Jiyoung
Hong, Hyewon
Kim, Woeyeon
Bohnert, Hans Jürgen
Bressan, Ray Anthony
Park, Hyeongcheol
Yun, Daejin
Date
2011-04Permanent link to this record
http://hdl.handle.net/10754/561741
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An Arabidopsis thaliana mutant, nks1-1, exhibiting enhanced sensitivity to NaCl was identified in a screen of a T-DNA insertion population in the genetic background of Col-0 gl1 sos3-1. Analysis of the genome sequence in the region flanking the T-DNA left border indicated two closely linked mutations in the gene encoded at locus At4g30996. A second allele, nks1-2, was obtained from the Arabidopsis Biological Resource Center. NKS1 mRNA was detected in all parts of wild-type plants but was not detected in plants of either mutant, indicating inactivation by the mutations. Both mutations in NKS1 were associated with increased sensitivity to NaCl and KCl, but not to LiCl or mannitol. NaCl sensitivity was associated with nks1 mutations in Arabidopsis lines expressing either wild type or alleles of SOS1, SOS2 or SOS3. The NaCl-sensitive phenotype of the nks1-2 mutant was complemented by expression of a full-length NKS1 allele from the CaMV35S promoter. When grown in medium containing NaCl, nks1 mutants accumulated more Na+ than wild type and K +/Na+ homeostasis was perturbed. It is proposed NKS1, a plant-specific gene encoding a 19 kDa endomembrane-localized protein of unknown function, is part of an ion homeostasis regulation pathway that is independent of the SOS pathway. © 2011 Elsevier Ltd. All rights reserved.Citation
Choi, W., Baek, D., Oh, D.-H., Park, J., Hong, H., Kim, W.-Y., … Yun, D.-J. (2011). NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis. Phytochemistry, 72(4-5), 330–336. doi:10.1016/j.phytochem.2010.12.005Sponsors
This work was supported by Grants from the World Class University Program (Grant No. R32-10148) funded by the Ministry of Education, Science and Technology and the Biogreen 21 Program (Grant No. 20070301034030) of the Rural Development Administration in Korea. W.C. is supported by a scholarship from the Brain Korea 21 program of the Ministry of Education, Science and Technology.Publisher
Elsevier BVJournal
PhytochemistryPubMed ID
21227472ae974a485f413a2113503eed53cd6c53
10.1016/j.phytochem.2010.12.005
Scopus Count
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