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dc.contributor.authorKhraiwesh, Basel
dc.contributor.authorFattash, Isam
dc.contributor.authorArif, Muhammad Asif
dc.contributor.authorFrank, Wolfgang
dc.date.accessioned2015-08-03T09:01:38Z
dc.date.available2015-08-03T09:01:38Z
dc.date.issued2011-04-15
dc.identifier.issn19406029
dc.identifier.pmid21533686
dc.identifier.doi10.1007/978-1-61779-123-9_5
dc.identifier.urihttp://hdl.handle.net/10754/561658
dc.description.abstractMicroRNAs (miRNAs) are ~21 nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. miRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest. During the last decade the moss Physcomitrella patens emerged as a model plant for functional gene analysis based on its unique ability to integrate DNA into the nuclear genome by homologous recombination which allows for the generation of targeted gene knockout mutants. In addition to this, we developed a protocol to express amiRNAs in P. patens that has particular advantages over the generation of knockout mutants and might be used to speed up reverse genetics approaches in this model species.
dc.publisherSpringer Nature
dc.titleGene function analysis by artificial microRNAs in Physcomitrella patens.
dc.typeArticle
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalRNAi and Plant Gene Function Analysis
kaust.personKhraiwesh, Basel
dc.date.published-online2011-04-15
dc.date.published-print2011


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