Diversity of Dominant Bacterial Taxa in Activated Sludge Promotes Functional Resistance following Toxic Shock Loading
KAUST DepartmentWater Desalination and Reuse Research Center (WDRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Environmental Biotechnology Research Group
Online Publication Date2010-12-14
Print Publication Date2011-04
Permanent link to this recordhttp://hdl.handle.net/10754/561641
MetadataShow full item record
AbstractExamining the relationship between biodiversity and functional stability (resistance and resilience) of activated sludge bacterial communities following disturbance is an important first step towards developing strategies for the design of robust biological wastewater treatment systems. This study investigates the relationship between functional resistance and biodiversity of dominant bacterial taxa by subjecting activated sludge samples, with different levels of biodiversity, to toxic shock loading with cupric sulfate (Cu[II]), 3,5-dichlorophenol (3,5-DCP), or 4-nitrophenol (4-NP). Respirometric batch experiments were performed to determine the functional resistance of activated sludge bacterial community to the three toxicants. Functional resistance was estimated as the 30 min IC50 or the concentration of toxicant that results in a 50% reduction in oxygen utilization rate compared to a referential state represented by a control receiving no toxicant. Biodiversity of dominant bacterial taxa was assessed using polymerase chain reaction-terminal restriction fragment length polymorphism (PCR-T-RFLP) targeting the 16S ribosomal RNA (16S rRNA) gene. Statistical analysis of 30 min IC50 values and PCR-T-RFLP data showed a significant positive correlation (P<0.05) between functional resistance and microbial diversity for each of the three toxicants tested. To our knowledge, this is the first study showing a positive correlation between biodiversity of dominant bacterial taxa in activated sludge and functional resistance. In this system, activated sludge bacterial communities with higher biodiversity are functionally more resistant to disturbance caused by toxic shock loading. © 2010 Springer Science+Business Media, LLC.
SponsorsThe authors thank Brian Kinkle, Bruce Rittmann, Makram Suidan, and Jim Young for useful discussion. Financial support from the National Science Foundation to Daniel B. Oerther (BES 0238858) is gratefully acknowledged.
- Molecular analysis of temporal changes of a bacterial community structure in activated sludge using denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH).
- Authors: Ziembińska A, Raszka A, Truu J, Surmacz-Górska J, Miksch K
- Issue date: 2007
- Support vector regression model of wastewater bioreactor performance using microbial community diversity indices: effect of stress and bioaugmentation.
- Authors: Seshan H, Goyal MK, Falk MW, Wuertz S
- Issue date: 2014 Apr 15
- A study of the relationship among sludge retention time, bacterial communities, and hydrolytic enzyme activities in inclined plate membrane bioreactors for the treatment of municipal wastewater.
- Authors: Ittisupornrat S, Tobino T, Yamamoto K
- Issue date: 2014 Nov
- Fine-scale bacterial community dynamics and the taxa-time relationship within a full-scale activated sludge bioreactor.
- Authors: Wells GF, Park HD, Eggleston B, Francis CA, Criddle CS
- Issue date: 2011 Nov 1
- Dynamics of dewaterability and bacterial populations in activated sludge.
- Authors: Li N, Satoh H, Mino T
- Issue date: 2012