Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea
KAUST DepartmentCenter for Desert Agriculture
Coastal and Marine Resources Core Lab
Computational Bioscience Research Center (CBRC)
Global Branding and Communications
OCRF- Special Academic Partnership
KAUST Grant NumberSA-C0040/UK-C0016
Online Publication Date2010-11-18
Print Publication Date2011-04
Permanent link to this recordhttp://hdl.handle.net/10754/561636
MetadataShow full item record
AbstractMarine sponges are associated with a remarkable array of microorganisms. Using a tag pyrosequencing technology, this study was the first to investigate in depth the microbial communities associated with three Red Sea sponges, Hyrtios erectus, Stylissa carteri and Xestospongia testudinaria. We revealed highly diverse sponge-associated bacterial communities with up to 1000 microbial operational taxonomic units (OTUs) and richness estimates of up to 2000 species. Altogether, 26 bacterial phyla were detected from the Red Sea sponges, 11 of which were absent from the surrounding sea water and 4 were recorded in sponges for the first time. Up to 100 OTUs with richness estimates of up to 300 archaeal species were revealed from a single sponge species. This is by far the highest archaeal diversity ever recorded for sponges. A non-negligible proportion of unclassified reads was observed in sponges. Our results demonstrated that the sponge-associated microbial communities remained highly consistent in the same sponge species from different locations, although they varied at different degrees among different sponge species. A significant proportion of the tag sequences from the sponges could be assigned to one of the sponge-specific clusters previously defined. In addition, the sponge-associated microbial communities were consistently divergent from those present in the surrounding sea water. Our results suggest that the Red Sea sponges possess highly sponge-specific or even sponge-species-specific microbial communities that are resistant to environmental disturbance, and much of their microbial diversity remains to be explored. © 2011 International Society for Microbial Ecology All rights reserved.
CitationLee, O. O., Wang, Y., Yang, J., Lafi, F. F., Al-Suwailem, A., & Qian, P.-Y. (2010). Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea. The ISME Journal, 5(4), 650–664. doi:10.1038/ismej.2010.165
SponsorsWe thank HC Chung, YH Wong, M Li and JP Ren from Hong Kong University of Science and Technology (HKUST), D Lau from Baptist University of Hong Kong and the technical team from King Abdullah University of Science and Technology (KAUST) for technical help during sample collection. We also thank Professor Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges, and HC Chung, K Cheng and YK Tam, HKUST, for assistance in nutrient analysis. We are grateful to Professor MW Taylor, University of Auckland, for providing an ARB database containing the alignment of all sponge-derived sequences for comparison. This publication is based on the work supported by an award (SA-C0040/UK-C0016) made by KAUST to P-Y Qian.
JournalThe ISME Journal
PubMed Central IDPMC3105750
- Specificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Sea.
- Authors: Moitinho-Silva L, Bayer K, Cannistraci CV, Giles EC, Ryu T, Seridi L, Ravasi T, Hentschel U
- Issue date: 2014 Mar
- Pyrosequencing reveals diverse and distinct sponge-specific microbial communities in sponges from a single geographical location in Irish waters.
- Authors: Jackson SA, Kennedy J, Morrissey JP, O'Gara F, Dobson AD
- Issue date: 2012 Jul
- Host-specific microbial communities in three sympatric North Sea sponges.
- Authors: Naim MA, Morillo JA, Sørensen SJ, Waleed AA, Smidt H, Sipkema D
- Issue date: 2014 Nov
- The putative functional ecology and distribution of archaeal communities in sponges, sediment and seawater in a coral reef environment.
- Authors: Polónia AR, Cleary DF, Freitas R, de Voogd NJ, Gomes NC
- Issue date: 2015 Jan
- Composition and Predictive Functional Analysis of Bacterial Communities in Seawater, Sediment and Sponges in the Spermonde Archipelago, Indonesia.
- Authors: Cleary DF, de Voogd NJ, Polónia AR, Freitas R, Gomes NC
- Issue date: 2015 Nov