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    Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred

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    Type
    Article
    Authors
    Holding, David R.
    Hunter, Brenda G.
    Klingler, John cc
    Wu, Song
    Guo, Xiaomei
    Gibbon, Bryan C.
    Wu., Rongling
    Schulze, Jan Michele
    Jung, Rudolf
    Larkins, Brian A.
    KAUST Department
    Desert Agriculture Initiative
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2010-11-13
    Online Publication Date
    2010-11-13
    Print Publication Date
    2011-03
    Permanent link to this record
    http://hdl.handle.net/10754/561634
    
    Metadata
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    Abstract
    Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway. © 2010 Springer-Verlag.
    Citation
    Holding, D. R., Hunter, B. G., Klingler, J. P., Wu, S., Guo, X., Gibbon, B. C., … Larkins, B. A. (2010). Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred. Theoretical and Applied Genetics, 122(4), 783–794. doi:10.1007/s00122-010-1486-3
    Sponsors
    The research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537), DOE (DE-FG02-96ER20242) and Pioneer Hi-Bred to BAL. We thank Roberto Lizzaraga-Guerra for contributing to the creation of the genetic materials that were used in this analysis.
    Publisher
    Springer Nature
    Journal
    Theoretical and Applied Genetics
    DOI
    10.1007/s00122-010-1486-3
    PubMed ID
    21076810
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00122-010-1486-3
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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