Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred
AuthorsHolding, David R.
Hunter, Brenda G.
Gibbon, Bryan C.
Schulze, Jan Michele
Larkins, Brian A.
KAUST DepartmentDesert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
MetadataShow full item record
AbstractQuality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway. © 2010 Springer-Verlag.
SponsorsThe research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537), DOE (DE-FG02-96ER20242) and Pioneer Hi-Bred to BAL. We thank Roberto Lizzaraga-Guerra for contributing to the creation of the genetic materials that were used in this analysis.
JournalTheoretical and Applied Genetics
- Genetic analysis of opaque2 modifier loci in quality protein maize.
- Authors: Holding DR, Hunter BG, Chung T, Gibbon BC, Ford CF, Bharti AK, Messing J, Hamaker BR, Larkins BA
- Issue date: 2008 Jul
- Deletion mutagenesis identifies a haploinsufficient role for γ-zein in opaque2 endosperm modification.
- Authors: Yuan L, Dou Y, Kianian SF, Zhang C, Holding DR
- Issue date: 2014 Jan
- Mapping QTLs for opaque2 modifiers influencing the tryptophan content in quality protein maize using genomic and candidate gene-based SSRs of lysine and tryptophan metabolic pathway.
- Authors: Babu BK, Agrawal PK, Saha S, Gupta HS
- Issue date: 2015 Jan
- Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize.
- Authors: Liu H, Shi J, Sun C, Gong H, Fan X, Qiu F, Huang X, Feng Q, Zheng X, Yuan N, Li C, Zhang Z, Deng Y, Wang J, Pan G, Han B, Lai J, Wu Y
- Issue date: 2016 May 3
- Transposable element rbg induces the differential expression of opaque-2 mutant gene in two maize o2 NILs derived from the same inbred line.
- Authors: Chen Y, Zhou Z, Zhao G, Li X, Song L, Yan N, Weng J, Hao Z, Zhang D, Li M, Zhang S
- Issue date: 2014