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dc.contributor.authorPamidimarri, D. V N N Sudheer
dc.contributor.authorMastan, Shaik G.
dc.contributor.authorRahman, Hifzur
dc.contributor.authorRavi Prakash, Ch
dc.contributor.authorSingh, Sweta V.
dc.contributor.authorReddy, Muppala P.
dc.date.accessioned2015-08-03T09:00:38Z
dc.date.available2015-08-03T09:00:38Z
dc.date.issued2010-07-30
dc.identifier.citationSudheer, P. D. V. N., Mastan, S. G., Rahman, H., Ravi Prakash, C., Singh, S., & Reddy, M. P. (2010). Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa. Molecular Biology Reports, 38(2), 1383–1388. doi:10.1007/s11033-010-0241-9
dc.identifier.issn03014851
dc.identifier.pmid20676774
dc.identifier.doi10.1007/s11033-010-0241-9
dc.identifier.urihttp://hdl.handle.net/10754/561625
dc.description.abstractThe present investigation was undertaken with an aim to check the ability of cross species amplification of microsatellite markers isolated from Jatropha curcas-a renewable source of biodiesel to deduce the generic relationship with its six sister taxa (J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica, and J. tanjorensis). Out of the 49 markers checked 31 markers showed cross species amplification in all the species studied. JCDS-30, JCDS-69, JCDS-26, JCMS-13 and JCMS-21 amplified in J. curcas. However, these markers did not show any cross species amplification. Overall percentage of polymorphism (PP) among the species studied was 38% and the mean genetic similarity (GS) was found to be 0.86. The highest PP (24) and least GS (0.76) was found between J. curcas/J. podagrica and J. curcas/J. multifida and least PP (4.44) and highest GS (0.96) was found between J. integerrima/J. tanjorensis. Dendrogram analysis showed good congruence to RAPD and AFLP than nrDNA ITS data reported earlier. The characterized microsatellites will pave way for intraspecies molecular characterization which can be further utilized in species differentiation, molecular identification, characterization of interspecific hybrids, exploitation of genetic resource management and genetic improvement of the species through marker assisted breeding for economically important traits. © 2010 Springer Science+Business Media B.V.
dc.description.sponsorshipWe are thankful to Council for Scientific and Industrial Research, New Delhi, India for its financial support and for research associate fellowship.
dc.publisherSpringer Nature
dc.subjectCross species amplification
dc.subjectGenetic relationship
dc.subjectJatropha curcas and microsatellites
dc.titleCross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa : Cross species amplification and genetic relationship of Jatropha using novel microsatellites
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.identifier.journalMolecular Biology Reports
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat 364002, India
kaust.personReddy, Muppala P.
dc.date.published-online2010-07-30
dc.date.published-print2011-02


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