Vertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencing
Lafi, Feras Fawzi
Al-Suwailem, Abdulaziz M.
KAUST DepartmentCenter for Desert Agriculture
Coastal and Marine Resources Core Lab
Desert Agriculture Initiative
KAUST Global Collaborative Research Program
OCRF- Special Academic Partnership
Online Publication Date2010-07-29
Print Publication Date2011-03
Permanent link to this recordhttp://hdl.handle.net/10754/561624
MetadataShow full item record
AbstractThe ecosystems of the Red Sea are among the least-explored microbial habitats in the marine environment. In this study, we investigated the microbial communities in the water column overlying the Atlantis II Deep and Discovery Deep in the Red Sea. Taxonomic classification of pyrosequencing reads of the 16S rRNA gene amplicons showed vertical stratification of microbial diversity from the surface water to 1500 m below the surface. Significant differences in both bacterial and archaeal diversity were observed in the upper (2 and 50 m) and deeper layers (200 and 1500 m). There were no obvious differences in community structure at the same depth for the two sampling stations. The bacterial community in the upper layer was dominated by Cyanobacteria whereas the deeper layer harbored a large proportion of Proteobacteria. Among Archaea, Euryarchaeota, especially Halobacteriales, were dominant in the upper layer but diminished drastically in the deeper layer where Desulfurococcales belonging to Crenarchaeota became the dominant group. The results of our study indicate that the microbial communities sampled in this study are different from those identified in water column in other parts of the world. The depth-wise compositional variation in the microbial communities is attributable to their adaptations to the various environments in the Red Sea. © 2011 International Society for Microbial Ecology All rights reserved.
SponsorsWe thank ML Sogin for his constructive comments on this article, the crew of the R/V Oceanus for providing technical assistance during the expedition and A Bower and S Swift of the Woods Hole Oceanographic Institution for providing environmental data and useful information of the study sites. We also thank R-L Liu of HKUST for the enumeration of microbial cells densities in water samples. This study was supported by an award (SA-C0040/UK-C0016) granted to PY Qian from the King Abdullah University of Science and Technology.
JournalThe ISME Journal
PubMed Central IDPMC3105721
- Archaeal and bacterial communities respond differently to environmental gradients in anoxic sediments of a California hypersaline lake, the Salton Sea.
- Authors: Swan BK, Ehrhardt CJ, Reifel KM, Moreno LI, Valentine DL
- Issue date: 2010 Feb
- Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea.
- Authors: Lee OO, Wang Y, Yang J, Lafi FF, Al-Suwailem A, Qian PY
- Issue date: 2011 Apr
- Changing composition of microbial communities indicates seepage fluid difference of the Thuwal Seeps in the Red Sea.
- Authors: Yang B, Zhang W, Tian R, Wang Y, Qian PY
- Issue date: 2015 Aug
- Pyrosequencing analysis of microbial communities reveals dominant cosmopolitan phylotypes in deep-sea sediments of the eastern Mediterranean Sea.
- Authors: Polymenakou PN, Christakis CA, Mandalakis M, Oulas A
- Issue date: 2015 Jun
- Distinctive microbial community structure in highly stratified deep-sea brine water columns.
- Authors: Bougouffa S, Yang JK, Lee OO, Wang Y, Batang Z, Al-Suwailem A, Qian PY
- Issue date: 2013 Jun