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dc.contributor.authorIdris, Ali
dc.contributor.authorTuttle, John Richard
dc.contributor.authorRobertson, Dominique Niki
dc.contributor.authorHaigler, Candace H.
dc.contributor.authorBrown, Judith K.
dc.date.accessioned2015-08-02T09:14:32Z
dc.date.available2015-08-02T09:14:32Z
dc.date.issued2010-08-04
dc.identifier.citationIdris, A. M., Tuttle, J. R., Robertson, D., Haigler, C. H., & Brown, J. K. (2010). Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. Physiological and Molecular Plant Pathology, 75(1-2), 13–22. doi:10.1016/j.pmpp.2010.07.002
dc.identifier.issn08855765
dc.identifier.doi10.1016/j.pmpp.2010.07.002
dc.identifier.urihttp://hdl.handle.net/10754/561574
dc.description.abstractA Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.
dc.publisherElsevier BV
dc.subjectBegomovirus
dc.subjectCotton breeding
dc.subjectCotton genetics
dc.subjectGeminivirus
dc.subjectGenetic improvement
dc.subjectMagnesium chelatase marker
dc.subjectViral episome
dc.titleDifferential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds
dc.typeArticle
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalPhysiological and Molecular Plant Pathology
dc.contributor.institutionSchool of Plant Sciences, University of Arizona, Tucson, AZ 85721, United States
dc.contributor.institutionDepartment of Plant Biology, North Carolina University, Raleigh, NC 27695, United States
dc.contributor.institutionDepartment of Crop Science, North Carolina University, Raleigh, NC 27695, United States
kaust.personIdris, Ali
dc.date.published-online2010-08-04
dc.date.published-print2010-12


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