Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Type
Article

Authors
Idris, Ali
Tuttle, John Richard
Robertson, Dominique Niki
Haigler, Candace H.
Brown, Judith K.

KAUST Department
Center for Desert Agriculture

Online Publication Date
2010-08-04

Print Publication Date
2010-12

Date
2010-08-04

Abstract
A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.

Citation
Idris, A. M., Tuttle, J. R., Robertson, D., Haigler, C. H., & Brown, J. K. (2010). Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. Physiological and Molecular Plant Pathology, 75(1-2), 13–22. doi:10.1016/j.pmpp.2010.07.002

Publisher
Elsevier BV

Journal
Physiological and Molecular Plant Pathology

DOI
10.1016/j.pmpp.2010.07.002

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