Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds
KAUST DepartmentDesert Agriculture Initiative
Online Publication Date2010-08-04
Print Publication Date2010-12
Permanent link to this recordhttp://hdl.handle.net/10754/561574
MetadataShow full item record
AbstractA Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.
CitationIdris, A. M., Tuttle, J. R., Robertson, D., Haigler, C. H., & Brown, J. K. (2010). Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. Physiological and Molecular Plant Pathology, 75(1-2), 13–22. doi:10.1016/j.pmpp.2010.07.002