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    Effect of nickel on regeneration in Jatropha curcas L. and assessment of genotoxicity using RAPD markers

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    Type
    Article
    Authors
    Sarkar, Tanmoy
    Vijay Anand, K. G.
    Reddy, Muppala P.
    KAUST Department
    Desert Agriculture Initiative
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2010-07-08
    Online Publication Date
    2010-07-08
    Print Publication Date
    2010-12
    Permanent link to this record
    http://hdl.handle.net/10754/561568
    
    Metadata
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    Abstract
    The aim of the present study was to determine the effect of nickel on shoot regeneration in tissue culture as well as to identify polymorphisms induced in leaf explants exposed to nickel through random amplified polymorphic DNA (RAPD). In vitro leaf explants of Jatropha curcas were grown in nickel amended Murashige and Skoog (MS) medium at four different concentrations (0, 0.01, 0.1, 1 mM) for 3 weeks. Percent regeneration, number of shoots produced and genotoxic effects were evaluated by RAPD using leaf explants obtained from the first three treatments following 5 weeks of their subsequent subculture in metal free MS medium. Percent regeneration decreased with increase in addition of nickel to the medium up to 14 days from 42.31% in control to zero in 1.0 mM. The number of shoot buds scored after 5 weeks was higher in control as compared to all other treatments except in one of the metal free subculture medium wherein the shoot number was higher in 0.01 mM treatment (mean = 7.80) than control (mean = 7.60). RAPD analysis produced only 5 polymorphic bands (3.225%) out of a total of 155 bands from 18 selected primers. Only three primers OPK-19, OPP-2, OPN-08 produced polymorphic bands. The dendrogram showed three groups A, B, and C. Group A samples showed 100% genetic similarity within them. Samples between groups B and C were more genetically distant from each other as compared to samples between groups A and B as well as groups A and C. Cluster analysis based on RAPD data correlated with treatments. © 2010 Springer Science+Business Media, LLC.
    Citation
    Sarkar, T., Vijay Anand, K. G., & Reddy, M. P. (2010). Effect of nickel on regeneration in Jatropha curcas L. and assessment of genotoxicity using RAPD markers. BioMetals, 23(6), 1149–1158. doi:10.1007/s10534-010-9364-7
    Publisher
    Springer Nature
    Journal
    BioMetals
    DOI
    10.1007/s10534-010-9364-7
    PubMed ID
    20614165
    ae974a485f413a2113503eed53cd6c53
    10.1007/s10534-010-9364-7
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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