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    The bifunctional abiotic stress signalling regulator and endogenous RNA silencing suppressor FIERY1 is required for lateral root formation

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    Type
    Article
    Authors
    Chen, Hao
    Xiong, Liming cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Desert Agriculture Initiative
    Plant Science
    Plant Stress Genomics Research Lab
    Date
    2010-09-28
    Online Publication Date
    2010-09-28
    Print Publication Date
    2010-12
    Permanent link to this record
    http://hdl.handle.net/10754/561567
    
    Metadata
    Show full item record
    Abstract
    The Arabidopsis FIERY1 (FRY1) locus was originally identified as a negative regulator of stress-responsive gene expression and later shown to be required for suppression of RNA silencing. In this study we discovered that the FRY1 locus also regulates lateral root formation. Compared with the wild type, fry1 mutant seedlings generated significantly fewer lateral roots under normal growth conditions and also exhibited a dramatically reduced sensitivity to auxin in inducing lateral root initiation. Using transgenic plants that overexpress a yeast homolog of FRY1 that possesses only the 3', 5'-bisphosphate nucleotidase activity but not the inositol 1-phosphatase activity, we demonstrated that the lateral root phenotypes in fry1 result from loss of the nucleotidase activity. Furthermore, a T-DNA insertion mutant of another RNA silencing suppressor, XRN4 (but not XRN2 or XRN3), which is an exoribonuclease that is inhibited by the substrate of the FRY1 3', 5'-bisphosphate nucleotidase, exhibits similar lateral root defects. Although fry1 and xrn4 exhibited reduced sensitivity to ethylene, our experiments demonstrated that restoration of ethylene sensitivity in the fry1 mutant is not sufficient to rescue the lateral root phenotypes of fry1. Our results indicate that RNA silencing modulated by FRY1 and XRN4 plays an important role in shaping root architecture. © 2010 Blackwell Publishing Ltd.
    Sponsors
    We thank Dr. John Celenza for the cyc1ATDB:GUS plasmid and Dr. Jian-Kang Zhu for critical reading of the paper. This study was supported by National Science Foundation grant #0446359 and United States Department of Agriculture National Research Initiative competitive grant #2004-02111 (to L. Xiong).
    Publisher
    Wiley
    Journal
    Plant, Cell & Environment
    DOI
    10.1111/j.1365-3040.2010.02218.x
    PubMed ID
    20807376
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1365-3040.2010.02218.x
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Desert Agriculture Initiative

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