The bifunctional abiotic stress signalling regulator and endogenous RNA silencing suppressor FIERY1 is required for lateral root formation
Type
ArticleAuthors
Chen, HaoXiong, Liming

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionDesert Agriculture Initiative
Bioscience Program
Plant Stress Genomics Research Lab
Date
2010-09-28Permanent link to this record
http://hdl.handle.net/10754/561567
Metadata
Show full item recordAbstract
The Arabidopsis FIERY1 (FRY1) locus was originally identified as a negative regulator of stress-responsive gene expression and later shown to be required for suppression of RNA silencing. In this study we discovered that the FRY1 locus also regulates lateral root formation. Compared with the wild type, fry1 mutant seedlings generated significantly fewer lateral roots under normal growth conditions and also exhibited a dramatically reduced sensitivity to auxin in inducing lateral root initiation. Using transgenic plants that overexpress a yeast homolog of FRY1 that possesses only the 3', 5'-bisphosphate nucleotidase activity but not the inositol 1-phosphatase activity, we demonstrated that the lateral root phenotypes in fry1 result from loss of the nucleotidase activity. Furthermore, a T-DNA insertion mutant of another RNA silencing suppressor, XRN4 (but not XRN2 or XRN3), which is an exoribonuclease that is inhibited by the substrate of the FRY1 3', 5'-bisphosphate nucleotidase, exhibits similar lateral root defects. Although fry1 and xrn4 exhibited reduced sensitivity to ethylene, our experiments demonstrated that restoration of ethylene sensitivity in the fry1 mutant is not sufficient to rescue the lateral root phenotypes of fry1. Our results indicate that RNA silencing modulated by FRY1 and XRN4 plays an important role in shaping root architecture. © 2010 Blackwell Publishing Ltd.Sponsors
We thank Dr. John Celenza for the cyc1ATDB:GUS plasmid and Dr. Jian-Kang Zhu for critical reading of the paper. This study was supported by National Science Foundation grant #0446359 and United States Department of Agriculture National Research Initiative competitive grant #2004-02111 (to L. Xiong).Publisher
Wiley-BlackwellJournal
Plant, Cell & EnvironmentISSN
01407791PubMed ID
20807376ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-3040.2010.02218.x
Scopus Count
Related articles
- Genetic interaction of two abscisic acid signaling regulators, HY5 and FIERY1, in mediating lateral root formation.
- Authors: Chen H, Xiong L
- Issue date: 2011 Jan
- Arabidopsis FIERY1, XRN2, and XRN3 are endogenous RNA silencing suppressors.
- Authors: Gy I, Gasciolli V, Lauressergues D, Morel JB, Gombert J, Proux F, Proux C, Vaucheret H, Mallory AC
- Issue date: 2007 Nov
- A nucleotide metabolite controls stress-responsive gene expression and plant development.
- Authors: Chen H, Zhang B, Hicks LM, Xiong L
- Issue date: 2011
- FIERY1 regulates light-mediated repression of cell elongation and flowering time via its 3'(2'),5'-bisphosphate nucleotidase activity.
- Authors: Kim BH, von Arnim AG
- Issue date: 2009 Apr
- A novel fry1 allele reveals the existence of a mutant phenotype unrelated to 5'->3' exoribonuclease (XRN) activities in Arabidopsis thaliana roots.
- Authors: Hirsch J, Misson J, Crisp PA, David P, Bayle V, Estavillo GM, Javot H, Chiarenza S, Mallory AC, Maizel A, Declerck M, Pogson BJ, Vaucheret H, Crespi M, Desnos T, Thibaud MC, Nussaume L, Marin E
- Issue date: 2011 Feb 3