Supra-optimal expression of the cold-regulated OsMyb4 transcription factor in transgenic rice changes the complexity of transcriptional network with major effects on stress tolerance and panicle development
Bajic, Vladimir B.
De Los Reyes, Benildo G.
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Applied Mathematics and Computational Science Program
MetadataShow full item record
AbstractThe R2R3-type OsMyb4 transcription factor of rice has been shown to play a role in the regulation of osmotic adjustment in heterologous overexpression studies. However, the exact composition and organization of its underlying transcriptional network has not been established to be a robust tool for stress tolerance enhancement by regulon engineering. OsMyb4 network was dissected based on commonalities between the global chilling stress transcriptome and the transcriptome configured by OsMyb4 overexpression. OsMyb4 controls a hierarchical network comprised of several regulatory sub-clusters associated with cellular defense and rescue, metabolism and development. It regulates target genes either directly or indirectly through intermediary MYB, ERF, bZIP, NAC, ARF and CCAAT-HAP transcription factors. Regulatory sub-clusters have different combinations of MYB-like, GCC-box-like, ERD1-box-like, ABRE-like, G-box-like, as1/ocs/TGA-like, AuxRE-like, gibberellic acid response element (GARE)-like and JAre-like cis-elements. Cold-dependent network activity enhanced cellular antioxidant capacity through radical scavenging mechanisms and increased activities of phenylpropanoid and isoprenoid metabolic processes involving various abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), ethylene and reactive oxygen species (ROS) responsive genes. OsMyb4 network is independent of drought response element binding protein/C-repeat binding factor (DREB/CBF) and its sub-regulons operate with possible co-regulators including nuclear factor-Y. Because of its upstream position in the network hierarchy, OsMyb4 functions quantitatively and pleiotrophically. Supra-optimal expression causes misexpression of alternative targets with costly trade-offs to panicle development. © 2010 Blackwell Publishing Ltd.
SponsorsThis study was funded by the United States Department of Agriculture (USDA) National Research Initiative (2006-35604-1669) and Maine Agricultural and Forestry Experiment Station (Publication 3104). M.-R.P. was a recipient of the Korea Research Foundation Fellowship (KRF-2006-352-F00002). S.-J.Y. and M.-R.P. were supported by BioGreen 21 Program-RDA (20080401034024), Republic of Korea. We also thank Dr Yulin Jia and the two anonymous reviewers for their insightful suggestions.
JournalPlant, Cell & Environment
- Overexpression of an ERF transcription factor TSRF1 improves rice drought tolerance.
- Authors: Quan R, Hu S, Zhang Z, Zhang H, Zhang Z, Huang R
- Issue date: 2010 May 1
- Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress.
- Authors: Yun KY, Park MR, Mohanty B, Herath V, Xu F, Mauleon R, Wijaya E, Bajic VB, Bruskiewich R, de Los Reyes BG
- Issue date: 2010 Jan 25
- Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice.
- Authors: Ito Y, Katsura K, Maruyama K, Taji T, Kobayashi M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K
- Issue date: 2006 Jan
- In silico analysis of cis-acting regulatory elements in 5' regulatory regions of sucrose transporter gene families in rice (Oryza sativa Japonica) and Arabidopsis thaliana.
- Authors: Ibraheem O, Botha CE, Bradley G
- Issue date: 2010 Dec
- The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice.
- Authors: Hossain MA, Cho JI, Han M, Ahn CH, Jeon JS, An G, Park PB
- Issue date: 2010 Nov 15