Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites
KAUST DepartmentDesert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2010-03-11
Print Publication Date2010-12
Permanent link to this recordhttp://hdl.handle.net/10754/561563
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AbstractJatropha curcas L. belongs to family Euphorbiaceae, native to South America attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petrodiesel. Very few attempts were made to isolate novel microsatellite markers and assessment of the extent of genetic equilibrium and diversity that exists in J. curcas. Therefore, the present investigation was undertaken to isolate the novel microsatellites and access genetic equilibrium, diversity that exists among 44 diverse germplasm collected from distinct geographical areas in India using isolated microsatellites. The overall efficiency of the enrichment of microsatellite by dual probe in the present study found to be 54% and among the sequences obtained the percentage of sequences having suitable flanking regions for the primer designing was found to be 89.58%. The mean co-efficient of genetic similarity (CGS) was found to be 0.97. The overall diversity obtained by microsatellites was found to be low in comparison with the diversity reported by multilocus markers systems observed in earlier studies; however, the good allele polymorphism was observed. The overall dendrogram of microsatellite analysis resulted in random clustering of germplasm and not in accordance to geographical area of collection. The present study, diversity analysis using microsatellite markers concludes the low genetic diversity and genetic disequlibrium of J. curcas in India and will provide pavement for further intra-population studies on narrow geographical areas to understand the population genetic structure, phylogeography and molecular ecological studies. The germplasm characterized, and the microsatellite markers isolated and characterized in the present study can be employed efficiently in breeding programs for genetic improvement of the species through marker assisted selection and QTL analysis, for further genetic resource management and help in making the J. curcas as potential crop with superior agronomical traits. © 2010 Springer Science+Business Media B.V.
SponsorsThe authors wish to thank Council for Scientific and Industrial Research (CSIR), New Delhi, India for financial support and for the Research Associate fellowship.
JournalMolecular Biology Reports
- Molecular characterization and genetic diversity analysis of Jatropha curcas L. in India using RAPD and AFLP analysis.
- Authors: Pamidimarri DV, Mastan SG, Rahman H, Reddy MP
- Issue date: 2010 Jun
- Molecular characterization of intra-population variability of Jatropha curcas L. using DNA based molecular markers.
- Authors: Mastan SG, Sudheer PD, Rahman H, Ghosh A, Rathore MS, Ravi Prakash Ch, Chikara J
- Issue date: 2012 Apr
- Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa: cross species amplification and genetic relationship of Jatropha using novel microsatellites.
- Authors: Sudheer PD, Mastan SG, Rahman H, Prakash ChR, Singh S, Reddy MP
- Issue date: 2011 Feb
- Phylogeography and molecular diversity analysis of Jatropha curcas L. and the dispersal route revealed by RAPD, AFLP and nrDNA-ITS analysis.
- Authors: Sudheer Pamidimarri DV, Reddy MP
- Issue date: 2014 May
- High efficiency and reliability of inter-simple sequence repeats (ISSR) markers for evaluation of genetic diversity in Brazilian cultivated Jatropha curcas L. accessions.
- Authors: Grativol C, da Fonseca Lira-Medeiros C, Hemerly AS, Ferreira PC
- Issue date: 2011 Oct