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    Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites

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    Type
    Article
    Authors
    Pamidimarri, D. V N N Sudheer
    Rahman, Hifzur
    Mastan, Shaik G.
    Reddy, Muppala P.
    KAUST Department
    Desert Agriculture Initiative
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2010-03-11
    Online Publication Date
    2010-03-11
    Print Publication Date
    2010-12
    Permanent link to this record
    http://hdl.handle.net/10754/561563
    
    Metadata
    Show full item record
    Abstract
    Jatropha curcas L. belongs to family Euphorbiaceae, native to South America attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petrodiesel. Very few attempts were made to isolate novel microsatellite markers and assessment of the extent of genetic equilibrium and diversity that exists in J. curcas. Therefore, the present investigation was undertaken to isolate the novel microsatellites and access genetic equilibrium, diversity that exists among 44 diverse germplasm collected from distinct geographical areas in India using isolated microsatellites. The overall efficiency of the enrichment of microsatellite by dual probe in the present study found to be 54% and among the sequences obtained the percentage of sequences having suitable flanking regions for the primer designing was found to be 89.58%. The mean co-efficient of genetic similarity (CGS) was found to be 0.97. The overall diversity obtained by microsatellites was found to be low in comparison with the diversity reported by multilocus markers systems observed in earlier studies; however, the good allele polymorphism was observed. The overall dendrogram of microsatellite analysis resulted in random clustering of germplasm and not in accordance to geographical area of collection. The present study, diversity analysis using microsatellite markers concludes the low genetic diversity and genetic disequlibrium of J. curcas in India and will provide pavement for further intra-population studies on narrow geographical areas to understand the population genetic structure, phylogeography and molecular ecological studies. The germplasm characterized, and the microsatellite markers isolated and characterized in the present study can be employed efficiently in breeding programs for genetic improvement of the species through marker assisted selection and QTL analysis, for further genetic resource management and help in making the J. curcas as potential crop with superior agronomical traits. © 2010 Springer Science+Business Media B.V.
    Citation
    Sudheer, P. D. V. N., Rahman, H., Mastan, S. G., & Reddy, M. P. (2010). Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites. Molecular Biology Reports, 37(8), 3785–3793. doi:10.1007/s11033-010-0033-2
    Sponsors
    The authors wish to thank Council for Scientific and Industrial Research (CSIR), New Delhi, India for financial support and for the Research Associate fellowship.
    Publisher
    Springer Nature
    Journal
    Molecular Biology Reports
    DOI
    10.1007/s11033-010-0033-2
    PubMed ID
    20221701
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11033-010-0033-2
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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