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dc.contributor.authorKim, Hyungsae
dc.contributor.authorKim, Sungjin
dc.contributor.authorAbbasi, Nazia
dc.contributor.authorBressan, Ray Anthony
dc.contributor.authorYun, Daejin
dc.contributor.authorYoo, Sangdong
dc.contributor.authorKwon, SukYun
dc.contributor.authorChoi, Sangbong
dc.date.accessioned2015-08-02T09:14:16Z
dc.date.available2015-08-02T09:14:16Z
dc.date.issued2010-10-05
dc.identifier.citationKim, H.-S., Kim, S. J., Abbasi, N., Bressan, R. A., Yun, D.-J., Yoo, S.-D., … Choi, S.-B. (2010). The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis. The Plant Journal, 64(3), 524–535. doi:10.1111/j.1365-313x.2010.04346.x
dc.identifier.issn09607412
dc.identifier.pmid20807212
dc.identifier.doi10.1111/j.1365-313X.2010.04346.x
dc.identifier.urihttp://hdl.handle.net/10754/561562
dc.description.abstractDof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity. © 2010 Blackwell Publishing Ltd.
dc.description.sponsorshipWe thank Gyung-Tae Kim for the rev-6 seeds and Yul-Ho Kim for the SEM pictures. S.-B. Choi and S.-Y. Kwon were supported by Bio-Green21 (grant 20080401034045 from the Rural Development Administration), and S.-B. Choi (CG2151) and S.-Y. Kwon (CG2120-1) were supported by the Crop Functional Genomics Center funded by the Ministry of Education, Science and Technology, Korea. S.-D. Yoo is supported by NRF (2010-0016898). R.A. Bressan and D.-J. Yun are supported by the WCU program (grant R32-10148).
dc.publisherWiley
dc.subjectActivation tagging mutant
dc.subjectDof transcription factor
dc.subjectDof5.1
dc.subjectLeaf polarity
dc.subjectRevoluta
dc.titleThe DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalThe Plant Journal
dc.contributor.institutionDivision of Bioscience and Bioinformatics, Myongji University, Yongin, Kyunggi-do 449-728, South Korea
dc.contributor.institutionSchool of Biotechnology and Environmental Engineering, Myongji University, Yongin, Kyunggi-do 449-728, South Korea
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907-2010, United States
dc.contributor.institutionDivision of Applied Life Science, Graduate School of Gyeongsang, National University, Jinju 660701, South Korea
dc.contributor.institutionDepartment of Biological Science, College of Natural Science, SungKyunKwan University, Suwon, Kyunggi-do 440-746, South Korea
dc.contributor.institutionKorea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, South Korea
kaust.personBressan, Ray Anthony
dc.date.published-online2010-10-05
dc.date.published-print2010-11


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