The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Bressan, Ray Anthony
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Online Publication Date2010-10-05
Print Publication Date2010-11
Permanent link to this recordhttp://hdl.handle.net/10754/561562
MetadataShow full item record
AbstractDof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity. © 2010 Blackwell Publishing Ltd.
CitationKim, H.-S., Kim, S. J., Abbasi, N., Bressan, R. A., Yun, D.-J., Yoo, S.-D., … Choi, S.-B. (2010). The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis. The Plant Journal, 64(3), 524–535. doi:10.1111/j.1365-313x.2010.04346.x
SponsorsWe thank Gyung-Tae Kim for the rev-6 seeds and Yul-Ho Kim for the SEM pictures. S.-B. Choi and S.-Y. Kwon were supported by Bio-Green21 (grant 20080401034045 from the Rural Development Administration), and S.-B. Choi (CG2151) and S.-Y. Kwon (CG2120-1) were supported by the Crop Functional Genomics Center funded by the Ministry of Education, Science and Technology, Korea. S.-D. Yoo is supported by NRF (2010-0016898). R.A. Bressan and D.-J. Yun are supported by the WCU program (grant R32-10148).
JournalThe Plant Journal
- The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis.
- Authors: Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL
- Issue date: 2006 Oct
- MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells.
- Authors: Konishi M, Donner TJ, Scarpella E, Yanagisawa S
- Issue date: 2015 Jan
- Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana.
- Authors: Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H
- Issue date: 2009 Oct
- Genetic interactions between leaf polarity-controlling genes and ASYMMETRIC LEAVES1 and 2 in Arabidopsis leaf patterning.
- Authors: Fu Y, Xu L, Xu B, Yang L, Ling Q, Wang H, Huang H
- Issue date: 2007 May
- Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves.
- Authors: Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C
- Issue date: 2007 Jul