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    Shoot regeneration from cotyledonary leaf explants of jatropha curcas: A biodiesel plant

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    Type
    Article
    Authors
    Kumar, Nitish Chandramohana
    Vijay Anand, K. G.
    Reddy, Muppala P.
    KAUST Department
    Desert Agriculture Initiative
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2010-03-07
    Online Publication Date
    2010-03-07
    Print Publication Date
    2010-09
    Permanent link to this record
    http://hdl.handle.net/10754/561555
    
    Metadata
    Show full item record
    Abstract
    A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 lM kinetin (Kn), 4.5 lM BAP, and 5.5 lM a-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 lM BAP and 8.5 lM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing dif- ferent concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l-1 activated charcoal. Elongated shoot treated with 15 lM IBA, 5.7 lM IAA, and 11 lM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification. © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010.
    Citation
    Kumar, N., Vijay Anand, K. G., & Reddy, M. P. (2010). Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant. Acta Physiologiae Plantarum, 32(5), 917–924. doi:10.1007/s11738-010-0479-9
    Sponsors
    The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi, India for financial assistance. Statistical analysis support from Mr. Keyur Shah and Mr. Amritpal singh, Assistant professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India is thankfully acknowledged.
    Publisher
    Springer Nature
    Journal
    Acta Physiologiae Plantarum
    DOI
    10.1007/s11738-010-0479-9
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11738-010-0479-9
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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