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dc.contributor.authorPark, Hyeongcheol
dc.contributor.authorKim, Hun
dc.contributor.authorKoo, Sungcheol
dc.contributor.authorPark, Heejin
dc.contributor.authorCheong, Misun
dc.contributor.authorHong, Hyewon
dc.contributor.authorBaek, Dongwon
dc.contributor.authorChung, Woosik
dc.contributor.authorKim, Dohhoon
dc.contributor.authorBressan, Ray Anthony
dc.contributor.authorLee, Sangyeol
dc.contributor.authorBohnert, Hans Jürgen
dc.contributor.authorYun, Daejin
dc.date.accessioned2015-08-02T09:14:04Z
dc.date.available2015-08-02T09:14:04Z
dc.date.issued2010-06-18
dc.identifier.citationPARK, H. C., KIM, H., KOO, S. C., PARK, H. J., CHEONG, M. S., HONG, H., … YUN, D.-J. (2010). Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice. Plant, Cell & Environment, 33(11), 1923–1934. doi:10.1111/j.1365-3040.2010.02195.x
dc.identifier.issn01407791
dc.identifier.pmid20561251
dc.identifier.doi10.1111/j.1365-3040.2010.02195.x
dc.identifier.urihttp://hdl.handle.net/10754/561553
dc.description.abstractSumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation. © 2010 Blackwell Publishing Ltd.
dc.description.sponsorshipThis work was supported by grants from the Biogreen 21 Program (20080401034023) of the Rural Development Administration, the World Class University Program (R32-10148) and the Environmental Biotechnology National Core Research Center (Grant#: R15-2003-012-01002-00) funded by the Ministry of Education, Science and Technology in Korea.
dc.publisherWiley
dc.subjectOryza sativa
dc.subjectSIZ/PIAS-type SUMO E3-ligase
dc.subjectStress response
dc.subjectSUMO
dc.subjectSumoylation
dc.titleFunctional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalPlant, Cell & Environment
dc.contributor.institutionDivision of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, South Korea
dc.contributor.institutionCollege of Natural Resources and Life Science, Dong-A University, Busan, 604-714, South Korea
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907-2054, United States
dc.contributor.institutionDepartments of Plant Biology and of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
kaust.personBressan, Ray Anthony
dc.date.published-online2010-06-18
dc.date.published-print2010-11


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