Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice
Type
ArticleAuthors
Park, HyeongcheolKim, Hun
Koo, Sungcheol
Park, Heejin
Cheong, Misun
Hong, Hyewon
Baek, Dongwon
Chung, Woosik
Kim, Dohhoon
Bressan, Ray Anthony
Lee, Sangyeol
Bohnert, Hans Jürgen
Yun, Daejin
Date
2010-06-18Online Publication Date
2010-06-18Print Publication Date
2010-11Permanent link to this record
http://hdl.handle.net/10754/561553
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Show full item recordAbstract
Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation. © 2010 Blackwell Publishing Ltd.Citation
PARK, H. C., KIM, H., KOO, S. C., PARK, H. J., CHEONG, M. S., HONG, H., … YUN, D.-J. (2010). Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice. Plant, Cell & Environment, 33(11), 1923–1934. doi:10.1111/j.1365-3040.2010.02195.xSponsors
This work was supported by grants from the Biogreen 21 Program (20080401034023) of the Rural Development Administration, the World Class University Program (R32-10148) and the Environmental Biotechnology National Core Research Center (Grant#: R15-2003-012-01002-00) funded by the Ministry of Education, Science and Technology in Korea.Publisher
WileyJournal
Plant, Cell & EnvironmentPubMed ID
20561251ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-3040.2010.02195.x
Scopus Count
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