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    Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice

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    Type
    Article
    Authors
    Park, Hyeongcheol
    Kim, Hun
    Koo, Sungcheol
    Park, Heejin
    Cheong, Misun
    Hong, Hyewon
    Baek, Dongwon
    Chung, Woosik
    Kim, Dohhoon
    Bressan, Ray Anthony
    Lee, Sangyeol
    Bohnert, Hans Jürgen
    Yun, Daejin
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2010-06-18
    Online Publication Date
    2010-06-18
    Print Publication Date
    2010-11
    Permanent link to this record
    http://hdl.handle.net/10754/561553
    
    Metadata
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    Abstract
    Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation. © 2010 Blackwell Publishing Ltd.
    Citation
    PARK, H. C., KIM, H., KOO, S. C., PARK, H. J., CHEONG, M. S., HONG, H., … YUN, D.-J. (2010). Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice. Plant, Cell & Environment, 33(11), 1923–1934. doi:10.1111/j.1365-3040.2010.02195.x
    Sponsors
    This work was supported by grants from the Biogreen 21 Program (20080401034023) of the Rural Development Administration, the World Class University Program (R32-10148) and the Environmental Biotechnology National Core Research Center (Grant#: R15-2003-012-01002-00) funded by the Ministry of Education, Science and Technology in Korea.
    Publisher
    Wiley
    Journal
    Plant, Cell & Environment
    DOI
    10.1111/j.1365-3040.2010.02195.x
    PubMed ID
    20561251
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1365-3040.2010.02195.x
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division

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