Transcriptional Response of Rhodococcus aetherivorans I24 to Polychlorinated Biphenyl-Contaminated Sediments
Type
ArticleAuthors
Puglisi, EdoardoCahill, Matt J.
Lessard, Philip A.
Capri, Ettore
Sinskey, Anthony John
Archer, John A.C.

Boccazzi, Paolo
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionComputational Bioscience Research Center (CBRC)
Date
2010-04-06Online Publication Date
2010-04-06Print Publication Date
2010-10Permanent link to this record
http://hdl.handle.net/10754/561549
Metadata
Show full item recordAbstract
We used a microarray targeting 3,524 genes to assess the transcriptional response of the actinomycete Rhodococcus aetherivorans I24 in minimal medium supplemented with various substrates (e. g., PCBs) and in both PCB-contaminated and non-contaminated sediment slurries. Relative to the reference condition (minimal medium supplemented with glucose), 408 genes were upregulated in the various treatments. In medium and in sediment, PCBs elicited the upregulation of a common set of 100 genes, including gene-encoding chaperones (groEL), a superoxide dismutase (sodA), alkyl hydroperoxide reductase protein C (ahpC), and a catalase/peroxidase (katG). Analysis of the R. aetherivorans I24 genome sequence identified orthologs of many of the genes in the canonical biphenyl pathway, but very few of these genes were upregulated in response to PCBs or biphenyl. This study is one of the first to use microarrays to assess the transcriptional response of a soil bacterium to a pollutant under conditions that more closely resemble the natural environment. Our results indicate that the transcriptional response of R. aetherivorans I24 to PCBs, in both medium and sediment, is primarily directed towards reducing oxidative stress, rather than catabolism. © 2010 Springer Science+Business Media, LLC.Sponsors
This work was supported by IRG Marie Curie Grant "COMEHERE," contract No. 21634, and by the Cambridge-MIT Institute.Publisher
Springer NatureJournal
Microbial EcologyPubMed ID
20369357ae974a485f413a2113503eed53cd6c53
10.1007/s00248-010-9650-5
Scopus Count
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