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    Transcriptional Response of Rhodococcus aetherivorans I24 to Polychlorinated Biphenyl-Contaminated Sediments

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    Type
    Article
    Authors
    Puglisi, Edoardo
    Cahill, Matt J.
    Lessard, Philip A.
    Capri, Ettore
    Sinskey, Anthony John
    Archer, John A.C. cc
    Boccazzi, Paolo
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Computational Bioscience Research Center (CBRC)
    Date
    2010-04-06
    Online Publication Date
    2010-04-06
    Print Publication Date
    2010-10
    Permanent link to this record
    http://hdl.handle.net/10754/561549
    
    Metadata
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    Abstract
    We used a microarray targeting 3,524 genes to assess the transcriptional response of the actinomycete Rhodococcus aetherivorans I24 in minimal medium supplemented with various substrates (e. g., PCBs) and in both PCB-contaminated and non-contaminated sediment slurries. Relative to the reference condition (minimal medium supplemented with glucose), 408 genes were upregulated in the various treatments. In medium and in sediment, PCBs elicited the upregulation of a common set of 100 genes, including gene-encoding chaperones (groEL), a superoxide dismutase (sodA), alkyl hydroperoxide reductase protein C (ahpC), and a catalase/peroxidase (katG). Analysis of the R. aetherivorans I24 genome sequence identified orthologs of many of the genes in the canonical biphenyl pathway, but very few of these genes were upregulated in response to PCBs or biphenyl. This study is one of the first to use microarrays to assess the transcriptional response of a soil bacterium to a pollutant under conditions that more closely resemble the natural environment. Our results indicate that the transcriptional response of R. aetherivorans I24 to PCBs, in both medium and sediment, is primarily directed towards reducing oxidative stress, rather than catabolism. © 2010 Springer Science+Business Media, LLC.
    Sponsors
    This work was supported by IRG Marie Curie Grant "COMEHERE," contract No. 21634, and by the Cambridge-MIT Institute.
    Publisher
    Springer Nature
    Journal
    Microbial Ecology
    DOI
    10.1007/s00248-010-9650-5
    PubMed ID
    20369357
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00248-010-9650-5
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC)

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