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dc.contributor.authorYamauchi, Kazuo
dc.contributor.authorYamasaki, Shizuo
dc.contributor.authorTakahashi, Rui
dc.contributor.authorAsakura, Tetsuo
dc.date.accessioned2015-08-02T09:13:14Z
dc.date.available2015-08-02T09:13:14Z
dc.date.issued2010-07
dc.identifier.citationYamauchi, K., Yamasaki, S., Takahashi, R., & Asakura, T. (2010). Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1mm microcoil MAS NMR probehead. Solid State Nuclear Magnetic Resonance, 38(1), 27–30. doi:10.1016/j.ssnmr.2010.06.002
dc.identifier.issn09262040
dc.identifier.pmid20579856
dc.identifier.doi10.1016/j.ssnmr.2010.06.002
dc.identifier.urihttp://hdl.handle.net/10754/561515
dc.description.abstractConformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.
dc.description.sponsorshipThe authors acknowledge the financial support of SENTAN, JST for the NMR probehead developments. TA acknowledges support from a Grant-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Supports of Japan (18105007) and also the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The authors acknowledge Prof. Mike Williamson, University of Sheffield, UK for many suggestions and careful reading of our paper.
dc.publisherElsevier BV
dc.subjectFiber
dc.subjectMass-limited sample
dc.subjectProbehead
dc.subjectSilk fibroin
dc.titleMicroscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead
dc.typeArticle
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentCore Labs
dc.identifier.journalSolid State Nuclear Magnetic Resonance
dc.contributor.institutionDepartment of Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei-shi, Tokyo 184-8588, Japan
kaust.personYamauchi, Kazuo


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