Show simple item record

dc.contributor.authorChen, Hao
dc.contributor.authorXiong, Liming
dc.date.accessioned2015-08-02T09:12:49Z
dc.date.available2015-08-02T09:12:49Z
dc.date.issued2010-06-01
dc.identifier.issn00219258
dc.identifier.pmid20516080
dc.identifier.doi10.1074/jbc.M110.123661
dc.identifier.urihttp://hdl.handle.net/10754/561497
dc.description.abstractmyo-Inositol-1-phosphate synthase is a conserved enzyme that catalyzes the first committed and rate-limiting step in inositol biosynthesis. Despite its wide occurrence in all eukaryotes, the role of myo-inositol-1-phosphate synthase and de novo inositol biosynthesis in cell signaling and organism development has been unclear. In this study, we isolated loss-of-function mutants in the Arabidopsis MIPS1 gene from different ecotypes. It was found that all mips1 mutants are defective in embryogenesis, cotyledon venation patterning, root growth, and root cap development. The mutant roots are also agravitropic and have reduced basipetal auxin transport. mips1 mutants have significantly reduced levels of major phosphatidylinositols and exhibit much slower rates of endocytosis. Treatment with brefeldin A induces slower PIN2 protein aggregation in mips1, indicating altered PIN2 trafficking. Our results demonstrate that MIPS1 is critical for maintaining phosphatidylinositol levels and affects pattern formation in plants likely through regulation of auxin distribution. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.description.sponsorshipThis work was supported by National Science Foundation Grant 0446359 (to L. X.).
dc.publisherAmerican Society for Biochemistry & Molecular Biology (ASBMB)
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911297
dc.titlemyo-Inositol-1-phosphate synthase is required for polar auxin transport and organ development
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalJournal of Biological Chemistry
dc.identifier.pmcidPMC2911297
dc.contributor.institutionDonald Danforth Plant Science Center, St. Louis, MO 63132, United States
kaust.personXiong, Liming
dc.date.published-online2010-06-01
dc.date.published-print2010-07-30


This item appears in the following Collection(s)

Show simple item record