myo-Inositol-1-phosphate synthase is required for polar auxin transport and organ development
Type
ArticleAuthors
Chen, HaoXiong, Liming

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Desert Agriculture Initiative
Plant Science
Plant Stress Genomics Research Lab
Date
2010-06-01Online Publication Date
2010-06-01Print Publication Date
2010-07-30Permanent link to this record
http://hdl.handle.net/10754/561497
Metadata
Show full item recordAbstract
myo-Inositol-1-phosphate synthase is a conserved enzyme that catalyzes the first committed and rate-limiting step in inositol biosynthesis. Despite its wide occurrence in all eukaryotes, the role of myo-inositol-1-phosphate synthase and de novo inositol biosynthesis in cell signaling and organism development has been unclear. In this study, we isolated loss-of-function mutants in the Arabidopsis MIPS1 gene from different ecotypes. It was found that all mips1 mutants are defective in embryogenesis, cotyledon venation patterning, root growth, and root cap development. The mutant roots are also agravitropic and have reduced basipetal auxin transport. mips1 mutants have significantly reduced levels of major phosphatidylinositols and exhibit much slower rates of endocytosis. Treatment with brefeldin A induces slower PIN2 protein aggregation in mips1, indicating altered PIN2 trafficking. Our results demonstrate that MIPS1 is critical for maintaining phosphatidylinositol levels and affects pattern formation in plants likely through regulation of auxin distribution. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.Citation
Chen, H., & Xiong, L. (2010). myo-Inositol-1-phosphate Synthase Is Required for Polar Auxin Transport and Organ Development. Journal of Biological Chemistry, 285(31), 24238–24247. doi:10.1074/jbc.m110.123661Sponsors
This work was supported by National Science Foundation Grant 0446359 (to L. X.).Journal
Journal of Biological ChemistryPubMed ID
20516080PubMed Central ID
PMC2911297Additional Links
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911297ae974a485f413a2113503eed53cd6c53
10.1074/jbc.M110.123661
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