Do GnRH analogues directly affect human endometrial epithelial cell gene expression?
Bocca, Silvina Maria
Bajic, Vladimir B.
Oehninger, Sergio Carlos
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Applied Mathematics and Computational Science Program
Online Publication Date2010-03-04
Print Publication Date2010-05-01
Permanent link to this recordhttp://hdl.handle.net/10754/561483
MetadataShow full item record
AbstractWe examined whether Gonadotrophin-releasing hormone (GnRH) analogues [leuprolide acetate (LA) and ganirelix acetate (GA)] modulate gene expression in Ishikawa cells used as surrogate for human endometrial epithelial cells in vitro. The specific aims were: (i) to study the modulatory effect of GnRH analogues by RT-PCR [in the absence and presence of E2 and P4, and cyclic adenosine monophos-phate (cAMP)] on mRNA expression of genes modulated during the window of implantation in GnRH analogues/rFSH-treated assisted reproductive technology cycles including OPTINEURIN (OPTN), CHROMATIN MODIFYING PROTEIN (CHMP1A), PROSAPOSIN (PSAP), IGFBP-5 and SORTING NEXIN 7 (SNX7), and (ii) to analyze the 5'-flanking regions of such genes for the presence of putative steroid-response elements [estrogen-response elements (EREs) and P4-response element (PREs)]. Ishikawa cells were cytokeratin+/vimentin2 and expressed ERa,ERb, PR and GnRH-R proteins. At 6 and 24 h, neither LA nor GA alone had an effect on gene expression. GnRH analogues alone or following E2 and/or P4 co-incubation for 24 h also had no effect on gene expression, but P4 significantly increased expression of CHMP1A.E2 + P4 treatment for 4 days, alone or followed by GA, had no effect, but E2 + P4 treatment followed by LA significantly decreased IGFBP-5 expression. The addition of 8-Br cAMP did not modify gene expression, with the exception of IGFBP-5 that was significantly increased. The GnRH analogues did not modify intracellular cAMP levels. We identified conserved EREs for OPN, CHMP1A, SNX7 and PSAP and PREs for SNX7. We conclude that GnRH analogues appear not to have major direct effects on gene expression of human endo-metrial epithelial cells in vitro. © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: email@example.com.
SponsorsHoward & Georgeanna Jones Foundation for Reproductive Medicine and Eastern Virginia Medical School.
PublisherOxford University Press (OUP)
JournalMolecular Human Reproduction
- Gonadotropin releasing hormone analogue (GnRHa) alters the expression and activation of Smad in human endometrial epithelial and stromal cells.
- Authors: Luo X, Xu J, Chegini N
- Issue date: 2003 Dec 16
- GnRH antagonists may affect endometrial receptivity.
- Authors: Rackow BW, Kliman HJ, Taylor HS
- Issue date: 2008 May
- Effect of GnRH analogues on apoptosis and expression of Bcl-2, Bax, Fas and FasL proteins in endometrial epithelial cell cultures from patients with endometriosis and controls.
- Authors: Bilotas M, Barañao RI, Buquet R, Sueldo C, Tesone M, Meresman G
- Issue date: 2007 Mar
- Hormonal regulation of estrogen receptor alpha and beta gene expression in human granulosa-luteal cells in vitro.
- Authors: Chiang CH, Cheng KW, Igarashi S, Nathwani PS, Leung PC
- Issue date: 2000 Oct
- Mid-luteal endometrial intracrinology following controlled ovarian hyperstimulation involving use of a gonadotrophin releasing hormone antagonist.
- Authors: Vani S, McDonald SE, Williams AR, Mason JI, Thong KJ, Critchley HO
- Issue date: 2007 Nov